Recombinant Anti-CDC123 antibody [EP5861] (ab129101)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP5861] to CDC123
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CDC123 antibody [EP5861]
See all CDC123 primary antibodies -
Description
Rabbit monoclonal [EP5861] to CDC123 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, 293T, Caco 2, RAW264.7 and PC12 cell lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.15 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP5861 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa). |
Target
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Function
Required for S phase entry of the cell cycle. -
Tissue specificity
Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes with the highest expression in testis. -
Sequence similarities
Belongs to the CDC123 family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 8872 Human
- Entrez Gene: 98828 Mouse
- Entrez Gene: 116656 Rat
- SwissProt: O75794 Human
- SwissProt: Q8CII2 Mouse
- SwissProt: Q62834 Rat
- Unigene: 412842 Human
- Unigene: 181490 Mouse
see all -
Alternative names
- C10orf7 antibody
- CD123_HUMAN antibody
- CDC123 antibody
see all
Images
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All lanes : Anti-CDC123 antibody [EP5861] (ab129101) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : RAW 264.7 cell lysate
Lane 5 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa -
ab129101, at 1/10 dilution staining CDC123 in permeabilized HeLa cells by intracellular flow cytometry (red) or a Rabbit IgG negative control (green).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab129101 has not yet been referenced specifically in any publications.