Anti-Cdc13 antibody [6F11/2] (ab10873)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-Cdc13 antibody [6F11/2]
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DescriptionMouse monoclonal [6F11/2] to Cdc13
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Host speciesMouse
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Tested applicationsSuitable for: WB, IPmore details
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Species reactivityReacts with: Schizosaccharomyces pombe
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Immunogen
Full length native protein (purified).
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General notes
Cdc13 is a fission yeast (S. pombe) B-type M-phase cyclin. Cdc13 binds to Cdk1 (cdc2), and the resulting Cdk1-Cdc13 complex controls the G2/M transition of the cell cycle. 6F11/2 can be used for detecting Cdc13 and associated Cdk1 kinase activation.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferConstituent: PBS
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Concentration information loading... -
PurityProtein A purified
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Primary antibody notesCdc13 is a fission yeast (S. pombe) B-type M-phase cyclin. Cdc13 binds to Cdk1 (cdc2), and the resulting Cdk1-Cdc13 complex controls the G2/M transition of the cell cycle. 6F11/2 can be used for detecting Cdc13 and associated Cdk1 kinase activation.
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ClonalityMonoclonal
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Clone number6F11/2
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MyelomaSp2
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IsotypeIgG2a
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab10873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent dilution. Predicted molecular weight: 48 kDa. | |
| IP | Use at an assay dependent dilution. |
Target
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Database links
- Entrez Gene: 2540880 Schizosaccharomyces pombe
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Alternative names
- CDC 13 antibody
- G2/mitotic specific cyclin antibody
Datasheets and documents
References
This product has been referenced in:
- Uchiyama M et al. The Protein Level of Rev1, a TLS Polymerase in Fission Yeast, Is Strictly Regulated during the Cell Cycle and after DNA Damage. PLoS One 10:e0130000 (2015). Read more (PubMed: 26147350) »
- Bandyopadhyay S et al. The basic leucine zipper domain transcription factor Atf1 directly controls Cdc13 expression and regulates mitotic entry independently of Wee1 and Cdc25 in Schizosaccharomyces pombe. Eukaryot Cell 13:813-21 (2014). Schizosaccharomyces pombe . Read more (PubMed: 24728197) »
