Anti-Cdc14B antibody (ab203675)
Key features and details
- Rabbit polyclonal to Cdc14B
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Cdc14B antibody -
Description
Rabbit polyclonal to Cdc14B -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide within Human Cdc14B aa 50-150 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O60729 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203675 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/500.
(or 1/50 - 1/200 if using a fluorescent secondary antibody) |
Notes |
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IHC-P
1/100 - 1/500. (or 1/50 - 1/200 if using a fluorescent secondary antibody) |
Target
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Function
Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily. -
Domain
Composed of two structurally equivalent A and B domains that adopt a dual specificity protein phosphatase (DSP) fold. -
Cellular localization
Nucleus > nucleolus. Nucleus > nucleoplasm. Following DNA damage, translocates from the nucleolus to the nucleoplasm and interacts with FZR1/CDH1. - Information by UniProt
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Database links
- Entrez Gene: 218294 Mouse
- SwissProt: Q6PFY9 Mouse
- Unigene: 25335 Mouse
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Alternative names
- CC14B_HUMAN antibody
- Cdc 14B antibody
- Cdc 14B1 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab203675 has been referenced in 1 publication.
- Subramanian GN et al. Oocytes mount a noncanonical DNA damage response involving APC-Cdh1-mediated proteolysis. J Cell Biol 219:N/A (2020). PubMed: 32328643