Anti-Cdc20 antibody (ab183479)
Key features and details
- Rabbit polyclonal to Cdc20
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Cdc20 antibody
See all Cdc20 primary antibodies -
Description
Rabbit polyclonal to Cdc20 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human Cdc20 aa 1-300.
Database link: Q12834 -
Positive control
- NIH 3T3 and INS1 cell lysates; U2OS cells transfected with Cdc20.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Predicted molecular weight: 55 kDa.
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IP |
Use at an assay dependent concentration.
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ICC/IF |
1/1000.
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Notes |
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WB
1/2000. Predicted molecular weight: 55 kDa. |
IP
Use at an assay dependent concentration. |
ICC/IF
1/1000. |
Target
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Function
Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the WD repeat CDC20/Fizzy family.
Contains 7 WD repeats. -
Developmental stage
Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition. -
Post-translational
modificationsPhosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.
Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C. - Information by UniProt
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Database links
- Entrez Gene: 991 Human
- Entrez Gene: 107995 Mouse
- Entrez Gene: 64515 Rat
- Omim: 603618 Human
- SwissProt: Q12834 Human
- SwissProt: Q9JJ66 Mouse
- SwissProt: Q62623 Rat
- Unigene: 524947 Human
see all -
Alternative names
- bA276H19.3 antibody
- Cdc 20 antibody
- CDC20 antibody
see all
Images
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All lanes : Anti-Cdc20 antibody (ab183479) at 1/2000 dilution
Lane 1 : U2OS cells transfected with control si RNA
Lane 2 : U2OS cells transfected with Cdc20 si RNA
Lane 3 : Rat INS1 cell lysate
Lane 4 : Mouse NIH 3T3 cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 55 kDa -
Western blot analysis of Immunoprecipitation using U2OS cells
Lane 1: Input.
Lane 2: control serum.
Lane 3: ab183479.
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Immunofluorescent analysis of U2OS cells showing labeling of Cdc20 with ab183479 at 1/1000 dilution. Anti-Cyclin B labeling is seen in red and DAPI counterstain in blue. Lower image is merged.
Protocols
Datasheets and documents
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Datasheet download
References (15)
ab183479 has been referenced in 15 publications.
- Song P et al. miR-200b/MYBL2/CDK1 suppresses proliferation and induces senescence through cell cycle arrest in ovine granulosa cells. Theriogenology 207:19-30 (2023). WB ; Sheep . PubMed: 37257219
- Song J et al. Proline-rich acidic protein 1 upregulates mitotic arrest deficient 1 to promote cisplatin-resistance of colorectal carcinoma by restraining mitotic checkpoint complex assembly. J Cancer 14:1515-1530 (2023). WB ; Human . PubMed: 37325046
- Peng W et al. The intercorrelation among CCT6A, CDC20, CCNB1, and PLK1 expressions and their clinical value in papillary thyroid carcinoma prognostication. J Clin Lab Anal 36:e24609 (2022). IHC ; Human . PubMed: 35838025
- Lan C et al. Inhibition of DYRK1A, via histone modification, promotes cardiomyocyte cell cycle activation and cardiac repair after myocardial infarction. EBioMedicine 82:104139 (2022). WB . PubMed: 35810562
- Wang Y et al. Phosphorylation of MAD2 at Ser195 Promotes Spindle Checkpoint Defects and Sensitizes Cancer Cells to Radiotherapy in ATM Deficient Cells. Front Cell Dev Biol 10:817831 (2022). PubMed: 35309941