Key features and details
- Rabbit polyclonal to Cdc20
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Cdc20 antibody
See all Cdc20 primary antibodies
DescriptionRabbit polyclonal to Cdc20
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide corresponding to a region between residues 50 and 100 of Human Cdc20 (NP_001246.2).
- Human Anaplastic Thyroid Carcinoma, Breast Carcinoma, Colon Carcinoma and Prostate Carcinoma tissues. Mouse teratoma tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRequired for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the WD repeat CDC20/Fizzy family.
Contains 7 WD repeats.
Developmental stageSynthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.
modificationsPhosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.
Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C.
- Information by UniProt
- bA276H19.3 antibody
- Cdc 20 antibody
- CDC20 antibody
ab86104, at 1/250 dilution, staining Cdc20 in formalin-fixed, paraffin-embedded human breast carcinoma tissue by Immunohistochemistry using DAB staining.
ab86104, at 1/250 dilution, staining Cdc20 in formalin-fixed, paraffin-embedded human colon carcinoma tissue by Immunohistochemistry using DAB staining.
ab86104, at 1/250 dilution, staining Cdc20 in formalin-fixed, paraffin-embedded mouse teratoma tissue by Immunohistochemistry using DAB staining.
ab86104 has been referenced in 3 publications.
- Huo X et al. Identification of prognosis markers for endometrial cancer by integrated analysis of DNA methylation and RNA-Seq data. Sci Rep 9:9924 (2019). PubMed: 31289358
- Mitsui Y et al. CYP1B1 promotes tumorigenesis via altered expression of CDC20 and DAPK1 genes in renal cell carcinoma. BMC Cancer 15:942 (2015). ICC/IF . PubMed: 26626260
- Genga KR et al. Proteins of the mitotic checkpoint and spindle are related to chromosomal instability and unfavourable prognosis in patients with myelodysplastic syndrome. J Clin Pathol 68:381-7 (2015). PubMed: 25637637