Overview

  • Product name

    Anti-Cdc20 antibody [CDC20/1102]
    See all Cdc20 primary antibodies
  • Description

    Mouse monoclonal [CDC20/1102] to Cdc20
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig
  • Immunogen

    Recombinant full length protein corresponding to Human Cdc20 aa 1-499.
    Sequence:

    MAQFAFESDL HSLLQLDAPI PNAPPARWQR KAKEAAGPAP SPMRAANRSH SAGRTPGRTP GKSSSKVQTT PSKPGGDRYI PHRSAAQMEV ASFLLSKENQ PENSQTPTKK EHQKAWALNL NGFDVEEAKI LRLSGKPQNA PEGYQNRLKV LYSQKATPGS SRKTCRYIPS LPDRILDAPE IRNDYYLNLV DWSSGNVLAV ALDNSVYLWS ASSGDILQLL QMEQPGEYIS SVAWIKEGNY LAVGTSSAEV QLWDVQQQKR LRNMTSHSAR VGSLSWNSYI LSSGSRSGHI HHHDVRVAEH HVATLSGHSQ EVCGLRWAPD GRHLASGGND NLVNVWPSAP GEGGWVPLQT FTQHQGAVKA VAWCPWQSNV LATGGGTSDR HIRIWNVCSG ACLSAVDAHS QVCSILWSPH YKELISGHGF AQNQLVIWKY PTMAKVAELK GHTSRVLSLT MSPDGATVAS AAADETLRLW RCFELDPARR REREKASAAK SSLIHQGIR


    Database link: Q12834

  • Positive control

    • Human gastric carcinoma and Human tonsil tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.05% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    ab215908 is purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    CDC20/1102
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab215908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells.

Target

  • Function

    Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the WD repeat CDC20/Fizzy family.
    Contains 7 WD repeats.
  • Developmental stage

    Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.
  • Post-translational
    modifications

    Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.
    Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C.
  • Information by UniProt
  • Database links

  • Alternative names

    • bA276H19.3 antibody
    • Cdc 20 antibody
    • CDC20 antibody
    • CDC20 cell division cycle 20 homolog antibody
    • CDC20_HUMAN antibody
    • CDC20A antibody
    • Cell division cycle 20 antibody
    • Cell division cycle 20 homolog (S. cerevisiae) antibody
    • Cell division cycle 20 homolog antibody
    • Cell division cycle protein 20 homolog antibody
    • fizzy antibody
    • MGC102824 antibody
    • p55CDC antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human tonsil tissue labeling Cdc20 with ab215908 at 4 μg/ml dilution.

  • Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human gastric carcinoma tissue labeling Cdc20 with ab215908 at 4 μg/ml dilution.

References

ab215908 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab215908.
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