Overview

  • Product name

  • Description

    Rabbit polyclonal to Cdc23
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 129-413 of Human Cdc23 (UniProt ID: Q9UJX2).

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2 and Raji whole cell lysates; A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab137461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 68 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the APC8/CDC23 family.
    Contains 9 TPR repeats.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation on Thr-562 occurs specifically during mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANAPC8 antibody
    • Anaphase promoting complex subunit 8 antibody
    • Anaphase-promoting complex subunit 8 antibody
    • Apc 8 antibody
    • APC8 antibody
    • Cdc 23 antibody
    • CDC23 antibody
    • CDC23_HUMAN antibody
    • cell division cycle 23 antibody
    • Cell division cycle 23 homolog antibody
    • Cell division cycle protein 23 antibody
    • Cell division cycle protein 23 homolog antibody
    • Cut23 antibody
    • Cyclosome subunit 8 antibody
    see all

Images

  • All lanes : Anti-Cdc23 antibody (ab137461) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 68 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde fixed A431 cells, labelling Cdc23 using ab137461 at 1/200 dilution. Right panel merged with DNA probe.

References

ab137461 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab137461.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up