Key features and details
- Rabbit polyclonal to Cdc23
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cdc23 antibody
See all Cdc23 primary antibodies
DescriptionRabbit polyclonal to Cdc23
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Xenopus tropicalis
Recombinant fragment corresponding to a region within amino acids 129-413 of Human Cdc23 (UniProt ID: Q9UJX2).
- 293T, A431, H1299, HeLa, HepG2 and Raji whole cell lysates; A431 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 68 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the APC8/CDC23 family.
Contains 9 TPR repeats.
modificationsPhosphorylated. Phosphorylation on Thr-562 occurs specifically during mitosis.
- Information by UniProt
- ANAPC8 antibody
- Anaphase promoting complex subunit 8 antibody
- Anaphase-promoting complex subunit 8 antibody
All lanes : Anti-Cdc23 antibody (ab137461) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 68 kDa
7.5% SDS PAGE
Immunofluorescence analysis of paraformaldehyde fixed A431 cells, labelling Cdc23 using ab137461 at 1/200 dilution. Right panel merged with DNA probe.
ab137461 has not yet been referenced specifically in any publications.