Overview

  • Product name
    Anti-Cdc23 antibody [EPR11827]
    See all Cdc23 primary antibodies
  • Description
    Rabbit monoclonal [EPR11827] to Cdc23
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human Cdc23 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9UJX2

  • Positive control
    • HepG2, HeLa, K562 and Jurkat cell lysates. HeLa cells.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 60 kDa (predicted molecular weight: 68 kDa).
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function
      Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Belongs to the APC8/CDC23 family.
      Contains 9 TPR repeats.
    • Post-translational
      modifications
      Phosphorylated. Phosphorylation on Thr-562 occurs specifically during mitosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • ANAPC8 antibody
      • Anaphase promoting complex subunit 8 antibody
      • Anaphase-promoting complex subunit 8 antibody
      • Apc 8 antibody
      • APC8 antibody
      • Cdc 23 antibody
      • CDC23 antibody
      • CDC23_HUMAN antibody
      • cell division cycle 23 antibody
      • Cell division cycle 23 homolog antibody
      • Cell division cycle protein 23 antibody
      • Cell division cycle protein 23 homolog antibody
      • Cut23 antibody
      • Cyclosome subunit 8 antibody
      see all

    Images

    • All lanes : Anti-Cdc23 antibody [EPR11827] (ab177148) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 68 kDa

    • Immunofluorescent analysis of HeLa cells labeling Cdc23 with ab177148 at 1/50 dilution.

    • Western blot analysis on immunoprecipitation pellet from (Lane 1) K562 cells lysate or (Lane 2) 1X PBS (negative control) using ab177148 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    ab177148 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab177148.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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