Product nameAnti-Cdc23 antibody [EPR11827]
See all Cdc23 primary antibodies
DescriptionRabbit monoclonal [EPR11827] to Cdc23
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Rat, Human
Synthetic peptide within Human Cdc23 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9UJX2
- HepG2, HeLa, K562 and Jurkat cell lysates. HeLa cells.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab177148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 60 kDa (predicted molecular weight: 68 kDa).|
|ICC/IF||1/50 - 1/100.|
|IP||1/10 - 1/100.|
FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the APC8/CDC23 family.
Contains 9 TPR repeats.
modificationsPhosphorylated. Phosphorylation on Thr-562 occurs specifically during mitosis.
- Information by UniProt
- ANAPC8 antibody
- Anaphase promoting complex subunit 8 antibody
- Anaphase-promoting complex subunit 8 antibody
All lanes : Anti-Cdc23 antibody [EPR11827] (ab177148) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 68 kDa
Immunofluorescent analysis of HeLa cells labeling Cdc23 with ab177148 at 1/50 dilution.
Western blot analysis on immunoprecipitation pellet from (Lane 1) K562 cells lysate or (Lane 2) 1X PBS (negative control) using ab177148 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab177148 has not yet been referenced specifically in any publications.