Overview

  • Product name

    Anti-Cdc23 antibody [EPR11828]
    See all Cdc23 primary antibodies
  • Description

    Rabbit monoclonal [EPR11828] to Cdc23
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Cdc23 aa 500 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9UJX2

  • Positive control

    • HepG2, HeLa, K562 and Jurkat cell lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab182003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 69 kDa).
ICC/IF 1/100.

Target

  • Function

    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the APC8/CDC23 family.
    Contains 9 TPR repeats.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation on Thr-562 occurs specifically during mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANAPC8 antibody
    • Anaphase promoting complex subunit 8 antibody
    • Anaphase-promoting complex subunit 8 antibody
    • Apc 8 antibody
    • APC8 antibody
    • Cdc 23 antibody
    • CDC23 antibody
    • CDC23_HUMAN antibody
    • cell division cycle 23 antibody
    • Cell division cycle 23 homolog antibody
    • Cell division cycle protein 23 antibody
    • Cell division cycle protein 23 homolog antibody
    • Cut23 antibody
    • Cyclosome subunit 8 antibody
    see all

Images

  • Immunofluorescent analysis of acetone-fixed HeLa cells labeling Cdc23 with ab182003 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution (Left panel). Counter stained with Dapi (Right panel).

  • All lanes : Anti-Cdc23 antibody [EPR11828] (ab182003) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 69 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Achari C  et al. Expression of miR-34c induces G2/M cell cycle arrest in breast cancer cells. BMC Cancer 14:538 (2014). WB ; Human . Read more (PubMed: 25064703) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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