Overview

  • Product name

    Anti-CDC25 (phospho S916) antibody
    See all CDC25 primary antibodies
  • Description

    Rabbit polyclonal to CDC25 (phospho S916)
  • Host species

    Rabbit
  • Specificity

    ab192816 detects endogenous levels of CDC25 only when phosphorylated at serine 916.
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human CDC25 conjugated to keyhole limpet haemocyanin. Peptide sequence around phosphorylation site of Serine 916 (N-E-G(p)-T P). NP_002882.3.
    Database link: Q13972

  • Positive control

    • Human brain tissue.
  • General notes

    Previously labelled as RASGRF1. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS, 0.87% Sodium chloride

    PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab192816 is purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.

Target

  • Function

    Promotes the exchange of Ras-bound GDP by GTP.
  • Sequence similarities

    Contains 1 DH (DBL-homology) domain.
    Contains 1 IQ domain.
    Contains 1 N-terminal Ras-GEF domain.
    Contains 2 PH domains.
    Contains 1 Ras-GEF domain.
  • Domain

    The DH (DBL-homology) domain mediates interaction with RASGRF2.
  • Post-translational
    modifications

    Ubiquitinated.
  • Information by UniProt
  • Database links

  • Alternative names

    • CDC25 antibody
    • CDC25L antibody
    • GNRP antibody
    • GRF1 antibody
    • GRF55 antibody
    • Guanine nucleotide exchange factor antibody
    • Guanine nucleotide releasing factor 55 kD antibody
    • Guanine nucleotide releasing protein antibody
    • guanine nucleotide-releasing factor 1 antibody
    • Guanine nucleotide-releasing protein antibody
    • H GRF55 antibody
    • HGRF55 antibody
    • PP13187 antibody
    • Ras protein specific guanine nucleotide releasing factor 1 antibody
    • Ras specific guanine nucleotide releasing factor antibody
    • Ras specific guanine nucleotide releasing factor CDC25 homolog antibody
    • Ras specific nucleotide exchange factor CDC25 antibody
    • Ras-GRF1 antibody
    • Ras-specific guanine nucleotide-releasing factor 1 antibody
    • Ras-specific nucleotide exchange factor CDC25 antibody
    • RASGRF1 antibody
    • RGRF1_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling CDC25 with ab192816 at a 1/50 dilution, the right panel was pre-incubated with blocking peptide.

References

ab192816 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab192816.
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