Our Abpromise guarantee covers the use of ab92892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    WB: 1/1000. Predicted molecular weight: 59 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
    • Sequence similarities

      Belongs to the MPI phosphatase family.
      Contains 1 rhodanese domain.
    • Domain

      The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.
    • Post-translational

      Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.
      Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cdc 25a antibody
      • CDC25A antibody
      • CDC25A2 antibody
      • CDC25A2 CAG isoform antibody
      • Cell division cycle 25 homolog A (S. pombe) antibody
      • Cell division cycle 25A antibody
      • Cell division cycle 25A isoform a antibody
      • Cell division cycle 25A isoform b antibody
      • D9Ertd393e antibody
      • Dual specificity phosphatase Cdc25A antibody
      • M phase inducer phosphatase 1 antibody
      • M-phase inducer phosphatase 1 antibody
      • MGC115549 antibody
      • MPIP1_HUMAN antibody
      see all


    • All lanes : Anti-Cdc25A antibody (ab92892) at 1/1000 dilution

      Lane 1 : HeLa cell lysate at 3 µg
      Lane 2 : HeLa cell lysate at 10 µg
      Lane 3 : HeLa cell lysate at 30 µg

      Predicted band size: 59 kDa


    ab92892 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As


    Thank you for contacting us. despite being provided in a 50% glycerol solution we would still recommend aliquoting this antibody prior to storing at -20°C. This avoids repeated freeze/thaw cycles and the detrimental effect this can have on the antibody’s binding capacity as well as any potential contamination of the antibody stock. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    For licensing inquiries, please contact partnerships@abcam.com

    Sign up