Anti-Cdc25A antibody (ab989)
Key features and details
- Rabbit polyclonal to Cdc25A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cdc25A antibody
See all Cdc25A primary antibodies -
Description
Rabbit polyclonal to Cdc25A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Cell Division Cycle 25a encoded by exon 7 (LocusLink ID 993).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab989 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
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| All applications |
Chimpanzee
Orangutan
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| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa). |
Target
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Function
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro. -
Sequence similarities
Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain. -
Domain
The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction. -
Post-translational
modificationsPhosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization. - Information by UniProt
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Database links
- Entrez Gene: 993 Human
- Omim: 116947 Human
- SwissProt: P30304 Human
- Unigene: 437705 Human
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Alternative names
- Cdc 25a antibody
- CDC25A antibody
- CDC25A2 antibody
see all
Images
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Western blot - Anti-Cdc25A antibody (ab989)
ab989 (Rabbit polyclonal to exon 7 of human Cdc25a) and ab991 (Rabbit polyclonal to exon 9 of human Cdc25a) both at a dilution of 1:2000, staining 500µg HeLa whole cell extract by Immunoprecipitation / Western Blot (ECL)
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab989 has been referenced in 10 publications.
- Das S et al. Genome-scale screening of deubiquitinase subfamily identifies USP3 as a stabilizer of Cdc25A regulating cell cycle in cancer. Cell Death Differ N/A:N/A (2020). PubMed: 32415280
- Wang Y et al. P53 suppresses SENP3 phosphorylation to mediate G2 checkpoint. Cell Discov 6:21 (2020). PubMed: 32351703
- Yin W et al. Circular RNA circ_0007142 Facilitates Colorectal Cancer Progression by Modulating CDC25A Expression via miR-122-5p. Onco Targets Ther 13:3689-3701 (2020). PubMed: 32431519
- Su J et al. Ultrasound-mediated destruction of vascular endothelial growth factor (VEGF) targeted and paclitaxel loaded microbubbles for inhibition of human breast cancer cell MCF-7 proliferation. Mol Cell Probes 46:101415 (2019). PubMed: 31228519
- Tu X et al. ATR Inhibition Is a Promising Radiosensitizing Strategy for Triple-Negative Breast Cancer. Mol Cancer Ther 17:2462-2472 (2018). PubMed: 30166399
- Martinez-Font E et al. Disruption of TCF/ß-Catenin Binding Impairs Wnt Signaling and Induces Apoptosis in Soft Tissue Sarcoma Cells. Mol Cancer Ther 16:1166-1176 (2017). PubMed: 28292937
- Zhang Z et al. Comprehensive analysis of differentially expressed genes associated with PLK1 in bladder cancer. BMC Cancer 17:861 (2017). PubMed: 29246203
- Liang J et al. PKM2 dephosphorylation by Cdc25A promotes the Warburg effect and tumorigenesis. Nat Commun 7:12431 (2016). WB ; Human . PubMed: 27485204
- Brunetto E et al. CDC25A protein stability represents a previously unrecognized target of HER2 signaling in human breast cancer: implication for a potential clinical relevance in trastuzumab treatment. Neoplasia 15:579-90 (2013). PubMed: 23730206
- Liu H et al. Phosphorylation of MLL by ATR is required for execution of mammalian S-phase checkpoint. Nature 467:343-6 (2010). WB ; Human . PubMed: 20818375