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    cdc25a-antibody-ab989.pdf

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Cell Biology Cell Cycle Kinases/Phosphatases Phosphatases
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Anti-Cdc25A antibody (ab989)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (10)

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Western blot - Anti-Cdc25A antibody (ab989)

    Key features and details

    • Rabbit polyclonal to Cdc25A
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
    Recombinant human Cdc25A protein (ab90763)

    View more associated products

    Overview

    • Product name

      Anti-Cdc25A antibody
      See all Cdc25A primary antibodies
    • Description

      Rabbit polyclonal to Cdc25A
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Cell Division Cycle 25a encoded by exon 7 (LocusLink ID 993).

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7
      Preservative: 0.1% Sodium azide
      Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Cell Cycle
      • Kinases/Phosphatases
      • Phosphatases
      • Epigenetics and Nuclear Signaling
      • Cell cycle
      • Kinases/Phosphatases
      • Phospatases
      • Cancer
      • Cell cycle
      • Kinases/phosphatases
      • Phosphatases

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human Cdc25A protein (ab90763)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab989 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Chimpanzee
    Orangutan
    Application Abreviews Notes
    WB (1)
    1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).
    Notes
    WB
    1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).

    Target

    • Function

      Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
    • Sequence similarities

      Belongs to the MPI phosphatase family.
      Contains 1 rhodanese domain.
    • Domain

      The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.
    • Post-translational
      modifications

      Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.
      Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.
    • Target information above from: UniProt accession P30304 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 993 Human
      • Omim: 116947 Human
      • SwissProt: P30304 Human
      • Unigene: 437705 Human
      • Alternative names

        • Cdc 25a antibody
        • CDC25A antibody
        • CDC25A2 antibody
        • CDC25A2 CAG isoform antibody
        • Cell division cycle 25 homolog A (S. pombe) antibody
        • Cell division cycle 25A antibody
        • Cell division cycle 25A isoform a antibody
        • Cell division cycle 25A isoform b antibody
        • D9Ertd393e antibody
        • Dual specificity phosphatase Cdc25A antibody
        • M phase inducer phosphatase 1 antibody
        • M-phase inducer phosphatase 1 antibody
        • MGC115549 antibody
        • MPIP1_HUMAN antibody
        see all

      Images

      • Western blot - Anti-Cdc25A antibody (ab989)
        Western blot - Anti-Cdc25A antibody (ab989)

        ab989 (Rabbit polyclonal to exon 7 of human Cdc25a) and ab991 (Rabbit polyclonal to exon 9 of human Cdc25a) both at a dilution of 1:2000, staining 500µg HeLa whole cell extract by Immunoprecipitation / Western Blot (ECL)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (10)

      Publishing research using ab989? Please let us know so that we can cite the reference in this datasheet.

      ab989 has been referenced in 10 publications.

      • Das S  et al. Genome-scale screening of deubiquitinase subfamily identifies USP3 as a stabilizer of Cdc25A regulating cell cycle in cancer. Cell Death Differ N/A:N/A (2020). PubMed: 32415280
      • Wang Y  et al. P53 suppresses SENP3 phosphorylation to mediate G2 checkpoint. Cell Discov 6:21 (2020). PubMed: 32351703
      • Yin W  et al. Circular RNA circ_0007142 Facilitates Colorectal Cancer Progression by Modulating CDC25A Expression via miR-122-5p. Onco Targets Ther 13:3689-3701 (2020). PubMed: 32431519
      • Su J  et al. Ultrasound-mediated destruction of vascular endothelial growth factor (VEGF) targeted and paclitaxel loaded microbubbles for inhibition of human breast cancer cell MCF-7 proliferation. Mol Cell Probes 46:101415 (2019). PubMed: 31228519
      • Tu X  et al. ATR Inhibition Is a Promising Radiosensitizing Strategy for Triple-Negative Breast Cancer. Mol Cancer Ther 17:2462-2472 (2018). PubMed: 30166399
      • Martinez-Font E  et al. Disruption of TCF/ß-Catenin Binding Impairs Wnt Signaling and Induces Apoptosis in Soft Tissue Sarcoma Cells. Mol Cancer Ther 16:1166-1176 (2017). PubMed: 28292937
      • Zhang Z  et al. Comprehensive analysis of differentially expressed genes associated with PLK1 in bladder cancer. BMC Cancer 17:861 (2017). PubMed: 29246203
      • Liang J  et al. PKM2 dephosphorylation by Cdc25A promotes the Warburg effect and tumorigenesis. Nat Commun 7:12431 (2016). WB ; Human . PubMed: 27485204
      • Brunetto E  et al. CDC25A protein stability represents a previously unrecognized target of HER2 signaling in human breast cancer: implication for a potential clinical relevance in trastuzumab treatment. Neoplasia 15:579-90 (2013). PubMed: 23730206
      • Liu H  et al. Phosphorylation of MLL by ATR is required for execution of mammalian S-phase checkpoint. Nature 467:343-6 (2010). WB ; Human . PubMed: 20818375

      Customer reviews and Q&As

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      Western blot abreview for Anti-Cdc25A antibody

      Average
      Abreviews
      Abreviews
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HT29 tumour cells)
      Loading amount
      50 µg
      Specification
      HT29 tumour cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
      Read More

      Mr. Paul Eve

      Verified customer

      Submitted Jan 09 2007

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