Other product details
Host species: Goat
Conjugation: Horse Radish Peroxidase
Also tested @ 1:100 and 1:200 with no pretreatments and with antigen retrival @ 110c for 2 minutes in EDTA buffer.
Weak nuclear staining with background wash.
Also tested several other xenografts where no staining was observed.
Option would have been to try longer antigen retrieval times including lower temperature (<110C). However, as the customer used an automated stainer, longer than 2 minutes retrieval resulted in tissue damage.
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Submitted Mar 17 2010