Product nameAnti-Cdc25A (phospho S124) antibody [EPR8888]
See all Cdc25A primary antibodies
DescriptionRabbit monoclonal [EPR8888] to Cdc25A (phospho S124)
Specificityab156574 only detects Cdc25A phosphorylated at Serine 124.
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Human
Synthetic phosphopeptide corresponding to residues surrounding Serine 124 of Human Cdc25A (UniProt ID: P30304).
- HeLa cell lysate; Human gastric carcinoma and Human testis tissues.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab156574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 59 kDa.|
|IHC-P||1/50 - 1/100.|
|IP||1/10 - 1/100.|
FunctionTyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Sequence similaritiesBelongs to the MPI phosphatase family.
Contains 1 rhodanese domain.
DomainThe phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.
modificationsPhosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.
- Information by UniProt
- Cdc 25a antibody
- CDC25A antibody
- CDC25A2 antibody
All lanes : Anti-Cdc25A (phospho S124) antibody [EPR8888] (ab156574) at 1/1000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Predicted band size: 59 kDa
ab156574, at a 1/1000 dilution, staining Cdc25A (phospho S124) in HeLa lysate immunoprecipitated using ab156574 at 1/10 dilution.
Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling Cdc25A (phospho S124) with ab156574 at 1/50 dilution.
Immunohistochemical analysis of paraffin embedded Human testis tissue labeling Cdc25A (phospho S124) with ab156574 at 1/50 dilution.
This product has been referenced in:
- Quan Wang J et al. Discovery of new chromen-4-one derivatives as telomerase inhibitors through regulating expression of dyskerin. J Enzyme Inhib Med Chem 33:1199-1211 (2018). Read more (PubMed: 30132373) »
- Brill E et al. Prexasertib, a cell cycle checkpoint kinases 1 and 2 inhibitor, increasesin vitrotoxicity of PARP inhibition by preventing Rad51 foci formation inBRCAwild type high-grade serous ovarian cancer. Oncotarget 8:111026-111040 (2017). Read more (PubMed: 29340034) »