Key features and details
- Rabbit polyclonal to Cdc25A (phospho T507)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Cdc25A (phospho T507) antibody
See all Cdc25A primary antibodies
DescriptionRabbit polyclonal to Cdc25A (phospho T507)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human lung carcinoma and mouse embryo tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.01% BSA, 50% Glycerol
Aqueous buffered solution
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.
(or 1/50 - 1/200 if using a fluorescent secondary antibody)
|WB||1/100 - 1/1000. Predicted molecular weight: 59 kDa.|
FunctionTyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Sequence similaritiesBelongs to the MPI phosphatase family.
Contains 1 rhodanese domain.
DomainThe phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.
modificationsPhosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.
- Information by UniProt
- Cdc 25a antibody
- CDC25A antibody
- CDC25A2 antibody
Immunohistochemical analysis of formalin-fixed paraffin-embedded human lung carcinoma tissue labeling Cdc25A (phospho T507) with ab203618 at 1/200, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed paraffin-embedded mouse embryo tissue labeling Cdc25A (phospho T507) with ab203618 at 1/200, followed by conjugation to the secondary antibody and DAB staining.
ab203618 has not yet been referenced specifically in any publications.