Overview

  • Product name

  • Description

    Mouse polyclonal to Cdc25B
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-580 of Human Cdc25B (NP_068659.1).

  • Positive control

    • Cdc25B transfected 293T cell line lysate. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab167347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 64 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
  • Sequence similarities

    Belongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.
  • Post-translational
    modifications

    Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.
  • Cellular localization

    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI604853 antibody
    • Cdc 25B antibody
    • Cdc25b antibody
    • CDC25HU2 antibody
    • Cdc25m2 antibody
    • Cell division cycle 25 homolog B antibody
    • Cell division cycle 25B antibody
    • Cell division cycle 25B isoform 1 antibody
    • Cell division cycle 25B isoform 2 antibody
    • Cell division cycle 25B isoform 3 antibody
    • Cell division cycle 25B isoform 4 antibody
    • Cell division cycle 25B isoform 5 antibody
    • Dual specificity phosphatase Cdc25B antibody
    • EC 3.1.3.48 antibody
    • M phase inducer phosphatase 2 antibody
    • M-phase inducer phosphatase 2 antibody
    • MPIP2_HUMAN antibody
    see all

Images

  • All lanes : Anti-Cdc25B antibody (ab167347) at 1 µg/ml

    Lane 1 : Cdc25B transfected 293T cell line lysate
    Lane 2 : Non-transfected 293T cell line lysate

    Lysates/proteins at 15 µl per lane.

    Predicted band size: 64 kDa

  • Immunofluorescent analysis of HeLa cells labeling Cdc25B with ab167347 at 10 µg/ml.

References

ab167347 has not yet been referenced specifically in any publications.

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