Key features and details
- Rabbit polyclonal to Cdc25C
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Cdc25C antibody
See all Cdc25C primary antibodies
DescriptionRabbit polyclonal to Cdc25C
Tested applicationsSuitable for: Flow Cyt, WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Human
- Mouse splenocytes; Mouse intestinal and testicular lysates.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab217920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).|
FunctionFunctions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
Sequence similaritiesBelongs to the MPI phosphatase family.
Contains 1 rhodanese domain.
Developmental stageExpressed predominantly in G2 phase.
modificationsPhosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.
- Information by UniProt
- CDC 25 antibody
- Cdc 25C antibody
- CDC25 antibody
All lanes : Anti-Cdc25C antibody (ab217920) at 1/300 dilution
Lane 1 : Mouse intestinal lysate
Lane 2 : Mouse testicular lysate
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
10% SDS-PAGE gel.
Flow cytometric analysis of mouse splenocytes labeling Cdc25C with ab217920 at 1/50 dilution for 30 minutes (green) followed by a FITC conjugated secondary antibody, compared to unstained cells (blue), secondary only (light blue) and isotype control(orange).
ab217920 has not yet been referenced specifically in any publications.