• Product name

  • Description

    Rabbit polyclonal to Cdc25C
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig, Chimpanzee
  • Immunogen

    Recombinant fragment within Human Cdc25C (internal sequence). The exact sequence is proprietary.
    Database link: P30307

  • Positive control

    • WB: RAW 264.7, Raji, PC-12, Rat2 and C2C12 whole cell extracts. IHC-P: Human SAS xenograft tissue. ICC/IF: U-2 OS cells.



Our Abpromise guarantee covers the use of ab226958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.


  • Function

    Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
  • Sequence similarities

    Belongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.
  • Developmental stage

    Expressed predominantly in G2 phase.
  • Post-translational

    Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • CDC 25 antibody
    • Cdc 25C antibody
    • CDC25 antibody
    • CDC25C antibody
    • Cell division cycle 25 homolog C antibody
    • Cell division cycle 25C antibody
    • Cell division cycle 25C protein antibody
    • Dual specificity phosphatase Cdc25C antibody
    • M phase inducer phosphatase 3 antibody
    • M-phase inducer phosphatase 3 antibody
    • Mitosis inducer CDC25 antibody
    • MPIP3 antibody
    • MPIP3_HUMAN antibody
    • Phosphotyrosine phosphatase antibody
    • PPP1R60 antibody
    • protein phosphatase 1, regulatory subunit 60 antibody
    see all


  • All lanes : Anti-Cdc25C antibody (ab226958) at 1/500 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    10% SDS-PAGE gel.

  • PFA-fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Cd25C (green) using ab226958 at 1/500 dilution in ICC/IF. Counterstain: alpha-tubulin filaments were labeled with an anti-alpha Tubulin antibody (red) at 1/2000 dilution.

  • Anti-Cdc25C antibody (ab226958) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

    10% SDS-PAGE gel.

  • Paraffin-embedded human SAS xenograft tissue stained for Cdc25C using ab226958 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-Cdc25C antibody (ab226958) at 1/1000 dilution

    Lane 1 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : C2C12 (mouse myoblast cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    10% SDS-PAGE gel.


This product has been referenced in:

  • Liu X  et al. Celastrol mediates autophagy and apoptosis via the ROS/JNK and Akt/mTOR signaling pathways in glioma cells. J Exp Clin Cancer Res 38:184 (2019). Read more (PubMed: 31053160) »
See 1 Publication for this product

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