Application
Flow Cytometry
Sample
Human Cell (HeLa Cells)
Specification
HeLa Cells
Preparation
Cell harvesting/tissue preparation method: Trypsin/EDTA
Sample buffer: Complete Media then PBS
Sample buffer: Complete Media then PBS
Fixation
Formaldehyde
Permeabilization
Yes - 90% Methanol
Other product details
Dilution
1/20
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: PBS + 10% Goat Serum
Secondary antibody
Name
Non-Abcam antibody was used: SCBT, Goat Anti-Rabbit FITC
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/1000
Additional data
Additional Notes
We stained 1,000,000 cells with parameters described above. We found that you could not store the cells prior to staining. The best results came when the cells were fixed, permeabilized, and stained all on the same day.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Brandon White
Verified customer
Submitted Aug 30 2012