Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cell lysate)
Loading amount
20 µg
Specification
HeLa cell lysate
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/5000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% BSA in TBS-T
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit IgG, HRP-linked antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
BioRad Clarity ECL
Exposure
30 second(s)
Bands
Specific: 57 kDa Non-specific: 53 kDa
Negative control
Untreated HeLa cell lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted May 15 2013