Overview

  • Product name

    Anti-Cdc25C antibody [E302] (HRP)
    See all Cdc25C primary antibodies
  • Description

    Rabbit monoclonal [E302] to Cdc25C (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human Cdc25C (N terminal). The exact sequence is proprietary.
    Database link: P30307

  • Positive control

    • WB: HeLa and wild-type HAP1 whole cell lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab205734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).

Target

  • Function

    Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
  • Sequence similarities

    Belongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.
  • Developmental stage

    Expressed predominantly in G2 phase.
  • Post-translational
    modifications

    Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CDC 25 antibody
    • Cdc 25C antibody
    • CDC25 antibody
    • CDC25C antibody
    • Cell division cycle 25 homolog C antibody
    • Cell division cycle 25C antibody
    • Cell division cycle 25C protein antibody
    • Dual specificity phosphatase Cdc25C antibody
    • M phase inducer phosphatase 3 antibody
    • M-phase inducer phosphatase 3 antibody
    • Mitosis inducer CDC25 antibody
    • MPIP3 antibody
    • MPIP3_HUMAN antibody
    • Phosphotyrosine phosphatase antibody
    • PPP1R60 antibody
    • protein phosphatase 1, regulatory subunit 60 antibody
    see all

Images

  • All lanes : Anti-Cdc25C antibody [E302] (HRP) (ab205734) at 1/2500 dilution

    Lane 1 : HeLa whole cell lysate (ab150035) at 10 µg
    Lane 2 : Wild-type HAP1 cell lysate at 20 µg
    Lane 3 : Cdc25C knockout HAP1 cell lysate at 20 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?


    Exposure time: 16 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205734 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab205734 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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