Product nameAnti-Cdc25C antibody [E302] (HRP)
See all Cdc25C primary antibodies
DescriptionRabbit monoclonal [E302] to Cdc25C (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rat
Synthetic peptide within Human Cdc25C (N terminal). The exact sequence is proprietary.
Database link: P30307
- WB: HeLa and wild-type HAP1 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.1% Proclin
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab205734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2500. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).|
FunctionFunctions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
Sequence similaritiesBelongs to the MPI phosphatase family.
Contains 1 rhodanese domain.
Developmental stageExpressed predominantly in G2 phase.
modificationsPhosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.
- Information by UniProt
- CDC 25 antibody
- Cdc 25C antibody
- CDC25 antibody
All lanes : Anti-Cdc25C antibody [E302] (HRP) (ab205734) at 1/2500 dilution
Lane 1 :
HeLa whole cell lysate (ab150035) at 10 µg
Lane 2 : Wild-type HAP1 cell lysate at 20 µg
Lane 3 : Cdc25C knockout HAP1 cell lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205734 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab205734 has not yet been referenced specifically in any publications.