Product nameAnti-Cdc25C antibody [E303]
See all Cdc25C primary antibodies
DescriptionRabbit monoclonal [E303] to Cdc25C
Specificityab32050 recognises Cdc25C. It does not cross react with other MPI phosphatase family members.
Tested applicationsSuitable for: WB, Flow Cyt, IPmore details
Unsuitable for: ICC/IF or IHC
Species reactivityReacts with: Human
Synthetic peptide within Human Cdc25C aa 450-550 (C terminal). The exact sequence is proprietary.
- A431 whole cell lysate (ab7909) can be used as a positive control in WB.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab32050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 53 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionFunctions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
Sequence similaritiesBelongs to the MPI phosphatase family.
Contains 1 rhodanese domain.
Developmental stageExpressed predominantly in G2 phase.
modificationsPhosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.
- Information by UniProt
- CDC 25 antibody
- Cdc 25C antibody
- CDC25 antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Cdc25C knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human bladder lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32050 observed at 58 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32050 was shown to specifically react with Cdc25C when Cdc25C knockout samples were used. Wild-type and Cdc25C knockout samples were subjected to SDS-PAGE. ab32050 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Anti-Cdc25C antibody [E303] (ab32050) at 1/1000 dilution + A431 cell lysate
Predicted band size: 53 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Han L et al. Long non-coding RNA SOX2OT promotes cell proliferation and motility in human ovarian cancer. Exp Ther Med 15:2182-2188 (2018). Read more (PubMed: 29434823) »
- Subramaniam D et al. Translation inhibition during cell cycle arrest and apoptosis: Mcl-1 is a novel target for RNA binding protein CUGBP2. Am J Physiol Gastrointest Liver Physiol 294:G1025-32 (2008). Read more (PubMed: 18292181) »