The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF: Use at a concentration of 2.5 µg/ml.
IP: Use at a concentration of 1 µg/sample.
WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 53 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
Belongs to the MPI phosphatase family. Contains 1 rhodanese domain.
Expressed predominantly in G2 phase.
Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.
protein phosphatase 1, regulatory subunit 60 antibody
Western blot - Anti-Cdc25C antibody [TC-14] (ab30313)
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: Cdc25C knockout HAP1 cell lysate (20 µg) Lane 3: HeLa cell lysate (20 µg) Lane 4: Hu bladder cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab30313 observed at 55 kDa. Red - loading control, ab181602, observed at 37 kDa. ab30313 was shown to recognize Cdc25C when Cdc25C knockout samples were used, along with additional cross-reactive bands. Wild-type and Cdc25C knockout samples were subjected to SDS-PAGE. ab30313 and ab181602 (loading control to GAPDH) were diluted at 0.5 µg/ml and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.