Anti-Cdc25C (phospho S216) antibody (ab47322)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-Cdc25C (phospho S216) antibody
See all Cdc25C primary antibodies -
Description
Rabbit polyclonal to Cdc25C (phospho S216) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of cdc25C only when phosphorylated at serine 216. -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human cdc25C around the phosphorylation site of serine 216 (S-P-SP-M-P).
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Positive control
- human breast carcinoma
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab47322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/200. | |
| WB | Use at an assay dependent concentration. | |
| IHC-P | 1/50 - 1/100. | |
| ELISA | 1/10000. |
Target
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Function
Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity. -
Sequence similarities
Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain. -
Developmental stage
Expressed predominantly in G2 phase. -
Post-translational
modificationsPhosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 995 Human
- Omim: 157680 Human
- SwissProt: P30307 Human
- Unigene: 656 Human
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Alternative names
- CDC 25 antibody
- Cdc 25C antibody
- CDC25 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25C (phospho S216) antibody (ab47322)ab47322 staining human breast carcinoma tissue by IHC-P (left hand panel). The right hand panel shows staining in the presence of phospho-peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-Cdc25C (phospho S216) antibody (ab47322)This image is courtesy of an Abreview submitted by Dr Kirk McManusab47322 detecting Cdc25C (phospho S216) in Hela Cells (green), together with a secondary goat anti-rabbit antibody conjugated to Cy3®. Cells were also stained with DAPI in order to highlight the nucleus/ chromosomes (red). Please refer to abreview for further details.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Peng B et al. Phosphorylation of proliferating cell nuclear antigen promotes cancer progression by activating the ATM/Akt/GSK3ß/Snail signaling pathway. J Biol Chem 294:7037-7045 (2019). Read more (PubMed: 30858175) »
- Liu X et al. Celastrol mediates autophagy and apoptosis via the ROS/JNK and Akt/mTOR signaling pathways in glioma cells. J Exp Clin Cancer Res 38:184 (2019). Read more (PubMed: 31053160) »
