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Cell Biology Cell Cycle Kinases/Phosphatases Phosphatases
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Anti-Cdc25C (phospho S216) antibody (ab47322)

  • Datasheet
Reviews (2) Submit a question References (4)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25C (phospho S216) antibody (ab47322)

    Key features and details

    • Rabbit polyclonal to Cdc25C (phospho S216)
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-Cdc25C (phospho S216) antibody
      See all Cdc25C primary antibodies
    • Description

      Rabbit polyclonal to Cdc25C (phospho S216)
    • Host species

      Rabbit
    • Specificity

      This antibody detects endogenous levels of cdc25C only when phosphorylated at serine 216.
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      The antiserum was produced against synthesized phosphopeptide derived from human cdc25C around the phosphorylation site of serine 216 (S-P-SP-M-P).

    • Positive control

      • human breast carcinoma
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Cell Cycle
      • Kinases/Phosphatases
      • Phosphatases
      • Epigenetics and Nuclear Signaling
      • Cell cycle
      • Kinases/Phosphatases
      • Phospatases

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Cdc25C protein (ab158076)

    Applications

    Our Abpromise guarantee covers the use of ab47322 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P 1/50 - 1/100.

    Target

    • Function

      Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
    • Sequence similarities

      Belongs to the MPI phosphatase family.
      Contains 1 rhodanese domain.
    • Developmental stage

      Expressed predominantly in G2 phase.
    • Post-translational
      modifications

      Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P30307 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 995 Human
      • Omim: 157680 Human
      • SwissProt: P30307 Human
      • Unigene: 656 Human
      • Alternative names

        • CDC 25 antibody
        • Cdc 25C antibody
        • CDC25 antibody
        • CDC25C antibody
        • Cell division cycle 25 homolog C antibody
        • Cell division cycle 25C antibody
        • Cell division cycle 25C protein antibody
        • Dual specificity phosphatase Cdc25C antibody
        • M phase inducer phosphatase 3 antibody
        • M-phase inducer phosphatase 3 antibody
        • Mitosis inducer CDC25 antibody
        • MPIP3 antibody
        • MPIP3_HUMAN antibody
        • Phosphotyrosine phosphatase antibody
        • PPP1R60 antibody
        • protein phosphatase 1, regulatory subunit 60 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25C (phospho S216) antibody (ab47322)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25C (phospho S216) antibody (ab47322)
        ab47322 staining human breast carcinoma tissue by IHC-P (left hand panel). The right hand panel shows staining in the presence of phospho-peptide.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (4)

      Publishing research using ab47322? Please let us know so that we can cite the reference in this datasheet.

      ab47322 has been referenced in 4 publications.

      • Peng B  et al. Phosphorylation of proliferating cell nuclear antigen promotes cancer progression by activating the ATM/Akt/GSK3ß/Snail signaling pathway. J Biol Chem 294:7037-7045 (2019). PubMed: 30858175
      • Liu X  et al. Celastrol mediates autophagy and apoptosis via the ROS/JNK and Akt/mTOR signaling pathways in glioma cells. J Exp Clin Cancer Res 38:184 (2019). PubMed: 31053160
      • Pan Z  et al. Cinobufagin Induces Cell Cycle Arrest at the G2/M Phase and Promotes Apoptosis in Malignant Melanoma Cells. Front Oncol 9:853 (2019). PubMed: 31552178
      • Wang Z  et al. Involvement of ATM-mediated Chk1/2 and JNK kinase signaling activation in HKH40A-induced cell growth inhibition. J Cell Physiol 221:213-20 (2009). WB ; Human . PubMed: 19530246

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-2 of 2 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cdc25C (phospho S216) antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Colon)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate buffer
      Permeabilization
      No
      Specification
      Colon
      Blocking step
      hydrogen peroxide as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More

      Vinicius Kannen Cardoso

      Verified customer

      Submitted Aug 10 2016

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Cdc25C (phospho S216) antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HeLa)
      Specification
      HeLa
      Fixative
      Paraformaldehyde
      Read More

      Dr. Kirk Mcmanus

      Verified customer

      Submitted Apr 24 2007

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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