Anti-Cdc26 / Apc12 antibody (ab96346)
Key features and details
- Rabbit polyclonal to Cdc26 / Apc12
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Cdc26 / Apc12 antibody -
Description
Rabbit polyclonal to Cdc26 / Apc12 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment, corresponding to a region within the N terminal amino acids 1-55 of Human Cdc26/ Apc12 (NP_644815)
-
Positive control
- 293T lysate; A431 cells for IF; H1299 whole cell lysate for WB
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 10 kDa.
|
|
ICC/IF |
1/100 - 1/200.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 10 kDa. |
ICC/IF
1/100 - 1/200. |
Target
-
Relevance
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc26, a component of cell cycle anaphase-promoting complex (APC). APC is composed of a group of highly conserved proteins and functions as a cell cycle-regulated ubiquitin-protein ligase. APC thus is responsible for the cell cycle regulated proteolysis of various proteins. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 777693 Cow
- Entrez Gene: 246184 Human
- Entrez Gene: 66440 Mouse
- Entrez Gene: 366381 Rat
- SwissProt: Q8NHZ8 Human
- SwissProt: Q99JP4 Mouse
- SwissProt: Q6YDN7 Rat
-
Alternative names
- ANAPC12 antibody
- Anaphase promoting complex subunit 12 antibody
- Anaphase promoting complex subunit CDC26 antibody
see all
Images
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab96346 has been referenced in 1 publication.
- Masuda K et al. LATS1 and LATS2 phosphorylate CDC26 to modulate assembly of the tetratricopeptide repeat subcomplex of APC/C. PLoS One 10:e0118662 (2015). PubMed: 25723520