Overview

  • Product name

    Anti-Cdc26 / Apc12 antibody
  • Description

    Rabbit polyclonal to Cdc26 / Apc12
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within the N terminal amino acids 1-55 of Human Cdc26/ Apc12 (NP_644815)

  • Positive control

    • 293T lysate; A431 cells for IF; H1299 whole cell lysate for WB

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 10 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Relevance

    The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc26, a component of cell cycle anaphase-promoting complex (APC). APC is composed of a group of highly conserved proteins and functions as a cell cycle-regulated ubiquitin-protein ligase. APC thus is responsible for the cell cycle regulated proteolysis of various proteins.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • ANAPC12 antibody
    • Anaphase promoting complex subunit 12 antibody
    • Anaphase promoting complex subunit CDC26 antibody
    • APC12 antibody
    • BWK 2 antibody
    • C9orf17 antibody
    • Cdc26 antibody
    • CDC26 subunit of anaphase promoting complex antibody
    • Cell division cycle 26 antibody
    • Cell division cycle 26 homolog (S. cerevisiae) antibody
    • Cell division cycle protein 26 homolog antibody
    • Chromosome 9 open reading frame 17 antibody
    • Hcn1 antibody
    • OTTMUSP00000015243 antibody
    • OTTMUSP00000015244 antibody
    • Protein BWK 2 antibody
    see all

Images

  • Anti-Cdc26 / Apc12 antibody (ab96346) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 10 kDa



    15% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed A431 cells, using ab96346 at 1/200 dilution. Lower image merged with DNA probe.

References

This product has been referenced in:

  • Masuda K  et al. LATS1 and LATS2 phosphorylate CDC26 to modulate assembly of the tetratricopeptide repeat subcomplex of APC/C. PLoS One 10:e0118662 (2015). Read more (PubMed: 25723520) »
See 1 Publication for this product

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