Overview

  • Product name

  • Description

    Rabbit polyclonal to Cdc27
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from the C-terminal region of Human Cdc27.

  • Positive control

    • Human Breast and Human Skin tissues, Mouse liver tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab113361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 92 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the APC3/CDC27 family.
    Contains 9 TPR repeats.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANAPC3 antibody
    • Anaphase Promoting Complex 3 antibody
    • Anaphase promoting complex protein 3 antibody
    • Anaphase Promoting Complex Subunit 3 antibody
    • Anaphase-promoting complex subunit 3 antibody
    • APC 3 antibody
    • APC3 antibody
    • Cdc 27 antibody
    • Cdc27 antibody
    • CDC27 homolog antibody
    • CDC27_HUMAN antibody
    • CDC27Hs antibody
    • Cell Division Cycle 27 antibody
    • Cell division cycle protein 27 homolog antibody
    • D0S1430E antibody
    • D17S978E antibody
    • H NUC antibody
    • H-NUC antibody
    • HNUC antibody
    • Nuc2 homolog antibody
    see all

Images

  • Lane 1 : Anti-Cdc27 antibody (ab113361) at 1 µg/ml
    Lane 2 : Anti-Cdc27 antibody (ab113361) at 2 µg/ml

    All lanes : Mouse liver tissue lysate

    Predicted band size: 92 kDa

  • Staining of Human Cdc27 in a Formalin/PFA-fixed paraffin-embedded section of Human Breast using ab113361 at a concentration of 5 µg/ml.
  • Staining of Human Cdc27 in a Formalin/PFA-fixed paraffin-embedded section of Human Skin using ab113361 at a concentration of 5 µg/ml.

References

ab113361 has not yet been referenced specifically in any publications.

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