Product nameAnti-Cdc27 antibody
See all Cdc27 primary antibodies
DescriptionRabbit polyclonal to Cdc27
Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chicken
- Hela whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab21386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/5000 - 1/40000.|
|WB||1/500 - 1/2000. Detects a band of approximately 90 kDa (predicted molecular weight: 92 kDa).|
|ICC/IF||Use at an assay dependent dilution.|
FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the APC3/CDC27 family.
Contains 9 TPR repeats.
modificationsPhosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.
- Information by UniProt
- ANAPC3 antibody
- Anaphase Promoting Complex 3 antibody
- Anaphase promoting complex protein 3 antibody
Anti-Cdc27 antibody (ab21386) at 1 µg/ml + Hela whole cell lysate at 35 µg
Goat anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 92 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
ab21386 has been referenced in 1 publication.
- Akimana C et al. Host factors required for modulation of phagosome biogenesis and proliferation of Francisella tularensis within the cytosol. PLoS One 5:e11025 (2010). WB ; Human . PubMed: 20552012