Key features and details
- Mouse monoclonal [AF 3.1] to Cdc27 - C-terminal
- Suitable for: WB, IHC-P, Flow Cyt, IP, IHC-Fr
- Reacts with: Xenopus laevis
- Isotype: IgG2b
Product nameAnti-Cdc27 antibody [AF 3.1] - C-terminal
See all Cdc27 primary antibodies
DescriptionMouse monoclonal [AF 3.1] to Cdc27 - C-terminal
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, IP, IHC-Frmore details
Species reactivityReacts with: Xenopus laevis
Predicted to work with: Mouse, Rat, Cow, Human, Mammals
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Clone numberAF 3.1
Our Abpromise guarantee covers the use of ab187817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 91 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the APC3/CDC27 family.
Contains 9 TPR repeats.
modificationsPhosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.
- Information by UniProt
- ANAPC3 antibody
- Anaphase Promoting Complex 3 antibody
- Anaphase promoting complex protein 3 antibody
ab187817 has not yet been referenced specifically in any publications.