Overview

  • Product name

    Anti-Cdc27 antibody [AF 3.1] - C-terminal
    See all Cdc27 primary antibodies
  • Description

    Mouse monoclonal [AF 3.1] to Cdc27 - C-terminal
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IP, IHC-Frmore details
  • Species reactivity

    Reacts with: Xenopus laevis
    Predicted to work with: Mouse, Rat, Cow, Human, Mammals
  • Immunogen

    Synthetic peptide corresponding to Human Cdc27 aa 815-824 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue).
    Sequence:

    QLHAAESDEF


    Database link: P30260

Properties

Applications

Our Abpromise guarantee covers the use of ab187817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 91 kDa.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function

    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the APC3/CDC27 family.
    Contains 9 TPR repeats.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANAPC3 antibody
    • Anaphase Promoting Complex 3 antibody
    • Anaphase promoting complex protein 3 antibody
    • Anaphase Promoting Complex Subunit 3 antibody
    • Anaphase-promoting complex subunit 3 antibody
    • APC 3 antibody
    • APC3 antibody
    • Cdc 27 antibody
    • Cdc27 antibody
    • CDC27 homolog antibody
    • CDC27_HUMAN antibody
    • CDC27Hs antibody
    • Cell Division Cycle 27 antibody
    • Cell division cycle protein 27 homolog antibody
    • D0S1430E antibody
    • D17S978E antibody
    • H NUC antibody
    • H-NUC antibody
    • HNUC antibody
    • Nuc2 homolog antibody
    see all

References

ab187817 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab187817.
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