Anti-Cdc27 antibody [AF3.1] (ab10538)
![Western blot - Anti-Cdc27 antibody [AF3.1] (ab10538)](https://www.abcam.com/ps/products/10/ab10538/Images/ab10538-18174-anti-cdc27-antibody-af31-western-blot.jpg "Western blot - Anti-Cdc27 antibody [AF3.1] (ab10538)")
Key features and details
- Mouse monoclonal [AF3.1] to Cdc27
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG2b
Overview
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Product name
Anti-Cdc27 antibody [AF3.1]
See all Cdc27 primary antibodies -
Description
Mouse monoclonal [AF3.1] to Cdc27 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB MouseHuman
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Immunogen
Synthetic peptide conjugated to KLH, corresponding to amino acids 814-823 of Human Cdc27.
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Positive control
- HeLa cell nuclear extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
AF3.1 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| IHC-P |
Human
|
| WB |
Mouse
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 92 kDa).
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 92 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
|
Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the APC3/CDC27 family.
Contains 9 TPR repeats. -
Post-translational
modificationsPhosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 996 Human
- Entrez Gene: 217232 Mouse
- Omim: 116946 Human
- SwissProt: P30260 Human
- SwissProt: A2A6Q5 Mouse
- Unigene: 463295 Human
- Unigene: 89845 Mouse
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Alternative names
- ANAPC3 antibody
- Anaphase Promoting Complex 3 antibody
- Anaphase promoting complex protein 3 antibody
see all
Images
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Western blot - Anti-Cdc27 antibody [AF3.1] (ab10538)All lanes : Anti-Cdc27 antibody [AF3.1] (ab10538)
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lane 3 : Mouse myeloid cell lysate
Predicted band size: 92 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
We have no more information on how this image was prepared by the academic who produced the antibody. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc27 antibody [AF3.1] (ab10538)](https://www.abcam.com/ps/products/10/ab10538/Images/ab10538-18173-anti-cdc27-antibody-af31-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc27 antibody [AF3.1] (ab10538)IHC image of ab10538 staining in human cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10538, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Flow Cytometry - Anti-Cdc27 antibody [AF3.1] (ab10538)](https://www.abcam.com/ps/products/10/ab10538/Images/ab10538-18172-anti-cdc27-antibody-af31-flow-cytometry.jpg)
Flow Cytometry - Anti-Cdc27 antibody [AF3.1] (ab10538)Overlay histogram showing K562 cells stained with ab10538 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10538, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
References (9)
ab10538 has been referenced in 9 publications.
- Wu Q et al. APC/C-CDH1-Regulated IDH3ß Coordinates with the Cell Cycle to Promote Cell Proliferation. Cancer Res 79:3281-3293 (2019). PubMed: 31053633
- Link LA et al. PCBP1/HNRNP E1 Protects Chromosomal Integrity by Translational Regulation of CDC27. Mol Cancer Res 14:634-46 (2016). PubMed: 27102006
- Scialpi F et al. EDD, a ubiquitin-protein ligase of the N-end rule pathway, associates with spindle assembly checkpoint components and regulates the mitotic response to nocodazole. J Biol Chem 290:12585-94 (2015). PubMed: 25833949
- Holt JE et al. Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes. Development 137:1297-304 (2010). WB, ICC/IF ; Mouse . PubMed: 20223764
- Ahlskog JK et al. Anaphase-promoting complex/cyclosome participates in the acute response to protein-damaging stress. Mol Cell Biol 30:5608-20 (2010). WB ; Human . PubMed: 20937767
- Kaur M et al. Mcm10 proteolysis initiates before the onset of M-phase. BMC Cell Biol 11:84 (2010). WB ; Human . PubMed: 20979666
- Mateo F et al. Degradation of cyclin A is regulated by acetylation. Oncogene 28:2654-66 (2009). WB ; Human . PubMed: 19483727
- Hochegger H et al. An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells. J Cell Biol 178:257-68 (2007). WB ; Chicken . PubMed: 17635936
- Yamano H et al. The role of the destruction box and its neighbouring lysine residues in cyclin B for anaphase ubiquitin-dependent proteolysis in fission yeast: defining the D-box receptor. EMBO J 17:5670-8 (1998). PubMed: 9755167
