Product nameAnti-Cdc27 antibody [AF3.1]
See all Cdc27 primary antibodies
DescriptionMouse monoclonal [AF3.1] to Cdc27
Tested applicationsSuitable for: IP, Other, WB, IHC-Fr, IHC-P, ELISA, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Cow, Dog, Human, Xenopus laevis
Synthetic peptide conjugated to KLH, corresponding to amino acids 814-823 of Human Cdc27.
- HeLa cell nuclear extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab10538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|Other||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 92 kDa).|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|AP||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the APC3/CDC27 family.
Contains 9 TPR repeats.
modificationsPhosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.
- Information by UniProt
- ANAPC3 antibody
- Anaphase Promoting Complex 3 antibody
- Anaphase promoting complex protein 3 antibody
All lanes : Anti-Cdc27 antibody [AF3.1] (ab10538)
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lane 3 : Mouse myeloid cell lysate
Predicted band size: 92 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
We have no more information on how this image was prepared by the academic who produced the antibody.
IHC image of ab10538 staining in human cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10538, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Overlay histogram showing K562 cells stained with ab10538 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10538, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This product has been referenced in:
- Link LA et al. PCBP1/HNRNP E1 Protects Chromosomal Integrity by Translational Regulation of CDC27. Mol Cancer Res 14:634-46 (2016). Read more (PubMed: 27102006) »
- Scialpi F et al. EDD, a ubiquitin-protein ligase of the N-end rule pathway, associates with spindle assembly checkpoint components and regulates the mitotic response to nocodazole. J Biol Chem 290:12585-94 (2015). Read more (PubMed: 25833949) »