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    cdc27-antibody-af31-ab10538.pdf

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Cell Biology Cell Cycle Cell Division Spindle
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Anti-Cdc27 antibody [AF3.1] (ab10538)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (9)

Product price, shipping and contact information

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Western blot - Anti-Cdc27 antibody [AF3.1] (ab10538)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc27 antibody [AF3.1] (ab10538)
  • Flow Cytometry - Anti-Cdc27 antibody [AF3.1] (ab10538)

Key features and details

  • Mouse monoclonal [AF3.1] to Cdc27
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Human
  • Isotype: IgG2b

You may also be interested in

Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-Cdc27 antibody [AF3.1]
    See all Cdc27 primary antibodies
  • Description

    Mouse monoclonal [AF3.1] to Cdc27
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 814-823 of Human Cdc27.

  • Positive control

    • HeLa cell nuclear extract.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    AF3.1
  • Isotype

    IgG2b
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • APC/C

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab10538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
IHC-P
Human
WB
Mouse
Human
Application Abreviews Notes
WB (1)
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 92 kDa).
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Notes
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 92 kDa).
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the APC3/CDC27 family.
    Contains 9 TPR repeats.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P30260 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 996 Human
    • Entrez Gene: 217232 Mouse
    • Omim: 116946 Human
    • SwissProt: P30260 Human
    • SwissProt: A2A6Q5 Mouse
    • Unigene: 463295 Human
    • Unigene: 89845 Mouse
    • Alternative names

      • ANAPC3 antibody
      • Anaphase Promoting Complex 3 antibody
      • Anaphase promoting complex protein 3 antibody
      • Anaphase Promoting Complex Subunit 3 antibody
      • Anaphase-promoting complex subunit 3 antibody
      • APC 3 antibody
      • APC3 antibody
      • Cdc 27 antibody
      • Cdc27 antibody
      • CDC27 homolog antibody
      • CDC27_HUMAN antibody
      • CDC27Hs antibody
      • Cell Division Cycle 27 antibody
      • Cell division cycle protein 27 homolog antibody
      • D0S1430E antibody
      • D17S978E antibody
      • H NUC antibody
      • H-NUC antibody
      • HNUC antibody
      • Nuc2 homolog antibody
      see all

    Images

    • Western blot - Anti-Cdc27 antibody [AF3.1] (ab10538)
      Western blot - Anti-Cdc27 antibody [AF3.1] (ab10538)
      All lanes : Anti-Cdc27 antibody [AF3.1] (ab10538)

      Lane 1 : HeLa cell lysate
      Lane 2 : 293 cell lysate
      Lane 3 : Mouse myeloid cell lysate

      Predicted band size: 92 kDa
      Observed band size: 80 kDa
      why is the actual band size different from the predicted?



      We have no more information on how this image was prepared by the academic who produced the antibody.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc27 antibody [AF3.1] (ab10538)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc27 antibody [AF3.1] (ab10538)
      IHC image of ab10538 staining in human cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10538, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Flow Cytometry - Anti-Cdc27 antibody [AF3.1] (ab10538)
      Flow Cytometry - Anti-Cdc27 antibody [AF3.1] (ab10538)
      Overlay histogram showing K562 cells stained with ab10538 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10538, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (9)

    Publishing research using ab10538? Please let us know so that we can cite the reference in this datasheet.

    ab10538 has been referenced in 9 publications.

    • Wu Q  et al. APC/C-CDH1-Regulated IDH3ß Coordinates with the Cell Cycle to Promote Cell Proliferation. Cancer Res 79:3281-3293 (2019). PubMed: 31053633
    • Link LA  et al. PCBP1/HNRNP E1 Protects Chromosomal Integrity by Translational Regulation of CDC27. Mol Cancer Res 14:634-46 (2016). PubMed: 27102006
    • Scialpi F  et al. EDD, a ubiquitin-protein ligase of the N-end rule pathway, associates with spindle assembly checkpoint components and regulates the mitotic response to nocodazole. J Biol Chem 290:12585-94 (2015). PubMed: 25833949
    • Holt JE  et al. Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes. Development 137:1297-304 (2010). WB, ICC/IF ; Mouse . PubMed: 20223764
    • Ahlskog JK  et al. Anaphase-promoting complex/cyclosome participates in the acute response to protein-damaging stress. Mol Cell Biol 30:5608-20 (2010). WB ; Human . PubMed: 20937767
    • Kaur M  et al. Mcm10 proteolysis initiates before the onset of M-phase. BMC Cell Biol 11:84 (2010). WB ; Human . PubMed: 20979666
    • Mateo F  et al. Degradation of cyclin A is regulated by acetylation. Oncogene 28:2654-66 (2009). WB ; Human . PubMed: 19483727
    • Hochegger H  et al. An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells. J Cell Biol 178:257-68 (2007). WB ; Chicken . PubMed: 17635936
    • Yamano H  et al. The role of the destruction box and its neighbouring lysine residues in cyclin B for anaphase ubiquitin-dependent proteolysis in fission yeast: defining the D-box receptor. EMBO J 17:5670-8 (1998). PubMed: 9755167

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-Cdc27 antibody [AF3.1]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Loading amount
    20 µg
    Specification
    HeLa
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 02 2009

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