Recombinant Anti-Cdc27 antibody [EPR7798] (ab129085)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7798] to Cdc27
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cdc27 antibody [EPR7798]
See all Cdc27 primary antibodies -
Description
Rabbit monoclonal [EPR7798] to Cdc27 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- K562, 293T, and HeLa whole cell lysate (ab150035)
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 9.80 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7798 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 91 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 91 kDa). |
Target
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Function
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the APC3/CDC27 family.
Contains 9 TPR repeats. -
Post-translational
modificationsPhosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 996 Human
- Entrez Gene: 217232 Mouse
- Entrez Gene: 360643 Rat
- Omim: 116946 Human
- SwissProt: P30260 Human
- SwissProt: A2A6Q5 Mouse
- SwissProt: Q4V8A2 Rat
- Unigene: 463295 Human
see all -
Alternative names
- ANAPC3 antibody
- Anaphase Promoting Complex 3 antibody
- Anaphase promoting complex protein 3 antibody
see all
Images
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All lanes : Anti-Cdc27 antibody [EPR7798] (ab129085) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 91 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab129085 has been referenced in 3 publications.
- Luong XG et al. Genome-wide analysis reveals a switch in the translational program upon oocyte meiotic resumption. Nucleic Acids Res 48:3257-3276 (2020). PubMed: 31970406
- Wu J et al. Overexpression of hsa-miR-186 induces chromosomal instability in arsenic-exposed human keratinocytes. Toxicol Appl Pharmacol 378:114614 (2019). PubMed: 31176655
- S Pedersen R et al. Profiling DNA damage response following mitotic perturbations. Nat Commun 7:13887 (2016). PubMed: 27976684