Overview

  • Product name

    Anti-Cdc27 antibody [EPR7798]
    See all Cdc27 primary antibodies
  • Description

    Rabbit monoclonal [EPR7798] to Cdc27
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Cdc27 (UniProt ID: P30260).

  • Positive control

    • K562, 293T, and HeLa whole cell lysate (ab150035)
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 91 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Belongs to the APC3/CDC27 family.
      Contains 9 TPR repeats.
    • Post-translational
      modifications

      Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • ANAPC3 antibody
      • Anaphase Promoting Complex 3 antibody
      • Anaphase promoting complex protein 3 antibody
      • Anaphase Promoting Complex Subunit 3 antibody
      • Anaphase-promoting complex subunit 3 antibody
      • APC 3 antibody
      • APC3 antibody
      • Cdc 27 antibody
      • Cdc27 antibody
      • CDC27 homolog antibody
      • CDC27_HUMAN antibody
      • CDC27Hs antibody
      • Cell Division Cycle 27 antibody
      • Cell division cycle protein 27 homolog antibody
      • D0S1430E antibody
      • D17S978E antibody
      • H NUC antibody
      • H-NUC antibody
      • HNUC antibody
      • Nuc2 homolog antibody
      see all

    Images

    • All lanes : Anti-Cdc27 antibody [EPR7798] (ab129085) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 91 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    See 1 Publication for this product

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