Product nameAnti-Cdc27 (phospho T244) antibody
See all Cdc27 primary antibodies
DescriptionRabbit polyclonal to Cdc27 (phospho T244)
Specificityab12281 is specific for the phosphorylated form of the Cdc27 (phospho T244). Less than 1% reactivity is observed against the non-phosphorylated form of the immunizing peptide. The antibody does not cross-react with Cdc27 phosphorylated at other sites.
Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Dog, Chimpanzee
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.
Our Abpromise guarantee covers the use of ab12281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF: Use at an assay dependent dilution.
WB: 1/300 - 1/2000. Detects a band of approximately 92 kDa.
ICC/IF: Use at an assay dependent dilution (from PubMed: 17472438).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the APC3/CDC27 family.
Contains 9 TPR repeats.
modificationsPhosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.
- Information by UniProt
- ANAPC3 antibody
- Anaphase Promoting Complex 3 antibody
- Anaphase promoting complex protein 3 antibody
All lanes : Anti-Cdc27 (phospho T244) antibody (ab12281) at 1/500 dilution
Lane 1 : HeLa whole cell lysate from asynchronous cells
Lane 2 : HeLa whole cell lysate from cells treated with nocodazole
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated Goat anti-Rabbit IgG [H&L] MX at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 92 kDa why is the actual band size different from the predicted?
Phosphorylated Cdc27 is mostly present only in cell preparations arrested in mitosis.
This product has been referenced in:
- Skoufias DA et al. Mitosis persists in the absence of Cdk1 activity when proteolysis or protein phosphatase activity is suppressed. J Cell Biol 179:671-85 (2007). WB ; Human . Read more (PubMed: 18025303) »
- Lindqvist A et al. Cyclin B1-Cdk1 activation continues after centrosome separation to control mitotic progression. PLoS Biol 5:e123 (2007). ICC/IF ; Human . Read more (PubMed: 17472438) »