Anti-Cdc37 antibody (ab115166)
Key features and details
- Rabbit polyclonal to Cdc37
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Cdc37 antibody
See all Cdc37 primary antibodies -
Description
Rabbit polyclonal to Cdc37 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
Immunogen
Synthetic peptide within Human Cdc37 aa 1-50. The exact sequence is proprietary.
Database link: Q16543 -
Positive control
- Hela, 293T and mpuse NIH3T3 cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab115166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 44 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 44 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity. -
Sequence similarities
Belongs to the CDC37 family. -
Post-translational
modificationsConstitutively sumoylated by UBE2I. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 101133417 Gorilla
- Entrez Gene: 11140 Human
- Entrez Gene: 12539 Mouse
- Entrez Gene: 114562 Rat
- Entrez Gene: 484955 Rhesus monkey
- Omim: 605065 Human
- SwissProt: Q16543 Human
- SwissProt: Q61081 Mouse
see all -
Alternative names
- CDC 37 antibody
- Cdc37 antibody
- CDC37 cell division cycle 37 homolog antibody
see all
Images
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All lanes : Anti-Cdc37 antibody (ab115166) at 0.4 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 cell lysate at 50 µg
Predicted band size: 44 kDa
Exposure time: 30 seconds -
Immunoprecipitation of Cdc37 (3 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with anti-Cdc37 antibody (ab115166); Lane 2-Immunoprecipitation with an anti-Cdc37 antibody that recognizes an downstream epitope ; Lane 3- Immunoprecipitation with IgG control. Anti-Cdc37 antibody (ab115166) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 10 sec.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab115166 has not yet been referenced specifically in any publications.