Product nameAnti-Cdc37 antibody
See all Cdc37 primary antibodies
DescriptionRabbit polyclonal to Cdc37
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide, corresponding to a region within amino acids 1 -50 of Human Cdc37 (Q16543).
- Hela, 293T and mpuse NIH3T3 cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab115166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 44 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionCo-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity.
Sequence similaritiesBelongs to the CDC37 family.
modificationsConstitutively sumoylated by UBE2I.
- Information by UniProt
- CDC 37 antibody
- Cdc37 antibody
- CDC37 cell division cycle 37 homolog antibody
All lanes : Anti-Cdc37 antibody (ab115166) at 0.4 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 cell lysate at 50 µg
Predicted band size: 44 kDa
Exposure time: 30 seconds
Immunoprecipitation of Cdc37 (3 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with anti-Cdc37 antibody (ab115166); Lane 2-Immunoprecipitation with an anti-Cdc37 antibody that recognizes an downstream epitope ; Lane 3- Immunoprecipitation with IgG control. Anti-Cdc37 antibody (ab115166) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 10 sec.
ab115166 has not yet been referenced specifically in any publications.