Overview

  • Product name

  • Description

    Rabbit polyclonal to Cdc37
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cynomolgus monkey, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide within Human Cdc37 aa 324-374. The exact sequence is proprietary. (NP_008996.1).
    Database link: Q16543

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab224831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 44 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity.
  • Sequence similarities

    Belongs to the CDC37 family.
  • Post-translational
    modifications

    Constitutively sumoylated by UBE2I.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • CDC 37 antibody
    • Cdc37 antibody
    • CDC37 cell division cycle 37 homolog antibody
    • CDC37 cell division cycle 37 S cerevisiae homolog antibody
    • CDC37 cell division cycle 37, S cerevisiae, homolog of antibody
    • Cdc37 homolog antibody
    • CDC37 protein antibody
    • CDC37_HUMAN antibody
    • CDC37A antibody
    • cell division cycle 37 antibody
    • Cell division cycle 37 homolog antibody
    • Hsp90 chaperone protein kinase targeting subunit antibody
    • Hsp90 chaperone protein kinase targeting subunit p50Cdc37 antibody
    • Hsp90 chaperone protein kinase-targeting subunit antibody
    • Hsp90 co chaperone Cdc37 antibody
    • Hsp90 co-chaperone Cdc37 antibody
    • p50 antibody
    • p50Cdc37 antibody
    • S cerevisiae hypothetical protein CDC37 antibody
    see all

Images

  • All lanes : Anti-Cdc37 antibody (ab224831) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 44 kDa


    Exposure time: 3 minutes
  • Cdc37 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab224831 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224831 at 1 µg/ml.

    Lane 1: ab224831 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 10 seconds.

References

ab224831 has not yet been referenced specifically in any publications.

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