Recombinant
RabMAb

Recombinant Anti-Cdc37 (phospho S13) antibody [EPR4879] (HRP) (ab202364)

Overview

  • Product name

    Anti-Cdc37 (phospho S13) antibody [EPR4879] (HRP)
    See all Cdc37 primary antibodies
  • Description

    Rabbit monoclonal [EPR4879] to Cdc37 (phospho S13) (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Cdc37 (phospho S13). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa whole cell lysate and HeLa whole cell lysate serum starved and treated with Calyculin A.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab202364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).

Target

  • Function

    Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity.
  • Sequence similarities

    Belongs to the CDC37 family.
  • Post-translational
    modifications

    Constitutively sumoylated by UBE2I.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • CDC 37 antibody
    • Cdc37 antibody
    • CDC37 cell division cycle 37 homolog antibody
    • CDC37 cell division cycle 37 S cerevisiae homolog antibody
    • CDC37 cell division cycle 37, S cerevisiae, homolog of antibody
    • Cdc37 homolog antibody
    • CDC37 protein antibody
    • CDC37_HUMAN antibody
    • CDC37A antibody
    • cell division cycle 37 antibody
    • Cell division cycle 37 homolog antibody
    • Hsp90 chaperone protein kinase targeting subunit antibody
    • Hsp90 chaperone protein kinase targeting subunit p50Cdc37 antibody
    • Hsp90 chaperone protein kinase-targeting subunit antibody
    • Hsp90 co chaperone Cdc37 antibody
    • Hsp90 co-chaperone Cdc37 antibody
    • p50 antibody
    • p50Cdc37 antibody
    • S cerevisiae hypothetical protein CDC37 antibody
    see all

Images

  • All lanes : Anti-Cdc37 (phospho S13) antibody [EPR4879] (HRP) (ab202364) at 1/5000 dilution

    Lane 1 : Untreated HeLa (Human epithelial carcinoma cell line) whole cell lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) whole cell lysate serum starved and Calyculin A treated
    Lane 3 : HeLa (Human epithelial carcinoma cell line) whole cell lysate, serum starved and Calyculin A treated. The membrane was then incubated with Alkaline phosphatase.

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 44 kDa
    Observed band size: 44 kDa


    Exposure time: 3 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab202864 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab202364 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab202364.
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