Overview

  • Product name

    Anti-Cdc4 / Fbw7 / hSel 10 antibody [FBOX 3a9/1]
    See all Cdc4 / Fbw7 / hSel 10 primary antibodies
  • Description

    Mouse monoclonal [FBOX 3a9/1] to Cdc4 / Fbw7 / hSel 10
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Cdc4/ Fbw7/ hSel 10.

Properties

Applications

Our Abpromise guarantee covers the use of ab74054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 80 kDa).
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100. PubMed: 20388706
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Relevance

    Fbw7 is a member of the F box protein family which are characterized by an approximately 40 amino acid motif, the F box. The F box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F box), which function in phosphorylation-dependent ubiquitination. The F box proteins are divided into 3 classes: Fbws containing WD40 domains, Fbls containing leucine rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. Fbw7 belongs to the Fbws class; in addition to an F box, this protein contains 7 tandem WD40 repeats. It binds directly to cyclin E and probably targets cyclin E for ubiquitin mediated degradation. Mutations of this gene are detected in ovarian and breast cancer cell lines. Alternative splicing of this gene generates 2 transcript variants diverging at the 5' termini.
  • Cellular localization

    Cytoplasmic and Nuclear
  • Database links

  • Alternative names

    • AGO antibody
    • Archipelago Drosophila homolog of antibody
    • Archipelago homolog antibody
    • CDC 4 antibody
    • CDC4 antibody
    • DKFZp686F23254 antibody
    • F box and WD 40 domain protein 7 antibody
    • F box protein FBW7 antibody
    • F box protein FBX30 antibody
    • F box protein SEL 10 antibody
    • F box/WD repeat protein 7 antibody
    • FBW 7 antibody
    • FBW6 antibody
    • FBW7 antibody
    • FBX 30 antibody
    • FBXO30 antibody
    • FBXW 6 antibody
    • FBXW 7 antibody
    • FBXW7 antibody
    • hAgo antibody
    • hCdc4 antibody
    • Homolog of C elegans sel 10 antibody
    • SEL10 antibody
    see all

Images

  • ab74054 at 1/100 dilution staining Cdc4 / Fbw7 / hSel 10 in human Hela cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed with 4% paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 1% bovine serum albumin in PBS for 1 h. A FITC-conjugated anti-mouse secondary antibody was used at 1/100 dilution. The nuclei were stained with 0.5 μg/ml 4′,6-diamidino-2-phenylindole. A mouse IgG was used as isotype control from other source. 

  • All lanes : Anti-Cdc4 / Fbw7 / hSel 10 antibody [FBOX 3a9/1] (ab74054) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 80 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?


    Exposure time: 16 minutes


    The 45 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Cdc4 / Fbw7 / hSel 10.
  • Overlay histogram showing HeLa cells stained with ab74054 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab74054, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:

  • Chen Y  et al. Promotional effect of microRNA-194 on breast cancer cells via targeting F-box/WD repeat-containing protein 7. Oncol Lett 15:4439-4444 (2018). WB ; Human . Read more (PubMed: 29541212) »
  • Priyadarshini R  et al. BLM Potentiates c-Jun Degradation and Alters Its Function as an Oncogenic Transcription Factor. Cell Rep 24:947-961.e7 (2018). Read more (PubMed: 30044990) »
See all 10 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
50 µg
Specification
293T
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 16 2012

Answer

Thank you for contacting Abcam

I am afraid that we do not have any information on the immunogen that was used and so I am unable to answer your question.

Apologies that I cannot help on this occasion. Should any further information come to light we will post that information on the webpage

Please let me know if there is anything else I can help you with.

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Answer

Thank you for contacting Abcam.


Regarding ab74054, the anti-Fbw7 antibody, I have checked with the laboratory and unfortunately we do not have any peptide or siRNA data available. The only IF data is that shown on our website.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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