Recombinant
RabMAb

Anti-CDC40 antibody [EPR12539] (ab175924)

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Overview

  • Product name
    Anti-CDC40 antibody [EPR12539]
    See all CDC40 primary antibodies
  • Description
    Rabbit Monoclonal [EPR12539] to CDC40
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CDC40 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: O60508

  • Positive control
    • HeLa, K562, 293T, HUVEC cell lysates, colon tissue, transitional cell carcinoma tissue, HeLa and K562 Kcells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab175924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 66 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IP.

    • Target

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC40 antibody [EPR12539] (ab175924)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC40 antibody [EPR12539] (ab175924)

      Immunohistochemical analysis of paraffin-embedded transitional cell carcinoma tissue labeling CDC40 with ab175924 at 1/50 dilution

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC40 antibody [EPR12539] (ab175924)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC40 antibody [EPR12539] (ab175924)

      Immunohistochemical analysis of paraffin-embedded colon tissue using labeling CDC40 with ab175924 at 1/50 dilution

    • Flow Cytometry - Anti-CDC40 antibody [EPR12539] (ab175924)
      Flow Cytometry - Anti-CDC40 antibody [EPR12539] (ab175924)

      Flow cytometric analysis of permeabilized K562 cells labeling CDC40 with ab175924 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    • Immunocytochemistry/ Immunofluorescence - Anti-CDC40 antibody [EPR12539] (ab175924)
      Immunocytochemistry/ Immunofluorescence - Anti-CDC40 antibody [EPR12539] (ab175924)

      Immunofluorescent staining of HeLa cells labeling CDC40 with ab175924 at 1/100 dilution.

    • Western blot - Anti-CDC40 antibody [EPR12539] (ab175924)
      Western blot - Anti-CDC40 antibody [EPR12539] (ab175924)
      All lanes : Anti-CDC40 antibody [EPR12539] (ab175924) at 1/1000 dilution

      Lane 1 : Hela cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HUVEC cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 66 kDa

    References

    ab175924 has not yet been referenced specifically in any publications.

    Publishing research using ab175924? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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