Anti-CDC40 antibody [EPR12539] Recombinant (ab175924)

Product name

Anti-CDC40 antibody [EPR12539]
See all CDC40 primary antibodies
Description

Rabbit monoclonal [EPR12539] to CDC40
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human CDC40 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: O60508
Positive control
- HeLa, K562, 293T, HUVEC cell lysates, colon tissue, transitional cell carcinoma tissue, HeLa and K562 Kcells
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12539
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab175924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/5000. Predicted molecular weight: 66 kDa.
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF		1/100 - 1/250.
Flow Cyt		1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes

Is unsuitable for IP.

Function

Associates with the spliceosome late in the splicing pathway and may function in the second step of pre-mRNA splicing.
Sequence similarities

Contains 7 WD repeats.
Cellular localization

Nucleus.
Target information above from: UniProt accession O60508 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 51362 Human
- Entrez Gene: 71713 Mouse
- Entrez Gene: 361859 Rat
- Omim: 605585 Human
- SwissProt: O60508 Human
- SwissProt: Q9DC48 Mouse
- Unigene: 428147 Human
- Unigene: 46063 Mouse
Alternative names
- Cdc40 antibody
- Cell division cycle 40 antibody
- Cell division cycle 40 homolog antibody
- EH binding protein 3 antibody
- EH-binding protein 3 antibody
- Ehb3 antibody
- hPRP17 antibody
- Pre mRNA processing factor 17 antibody
- Pre mRNA splicing factor 17 antibody
- Pre-mRNA-processing factor 17 antibody
- PRP17 antibody
- PRP17 homolog antibody
- PRP17_HUMAN antibody
- PRPF17 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC40 antibody [EPR12539] (ab175924)

Immunohistochemical analysis of paraffin-embedded transitional cell carcinoma tissue labeling CDC40 with ab175924 at 1/50 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC40 antibody [EPR12539] (ab175924)

Immunohistochemical analysis of paraffin-embedded colon tissue using labeling CDC40 with ab175924 at 1/50 dilution
Flow Cytometry - Anti-CDC40 antibody [EPR12539] (ab175924)

Flow cytometric analysis of permeabilized K562 cells labeling CDC40 with ab175924 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Immunocytochemistry/ Immunofluorescence - Anti-CDC40 antibody [EPR12539] (ab175924)

Immunofluorescent staining of HeLa cells labeling CDC40 with ab175924 at 1/100 dilution.
Western blot - Anti-CDC40 antibody [EPR12539] (ab175924)

All lanes : Anti-CDC40 antibody [EPR12539] (ab175924) at 1/1000 dilution

Lane 1 : Hela cell lysate
Lane 2 : K562 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HUVEC cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 66 kDa

ab175924 has not yet been referenced specifically in any publications.

Publishing research using ab175924? Please let us know so that we can cite the reference in this datasheet.

Anti-CDC40 antibody [EPR12539] (ab175924)