
Recombinant Anti-CDC40 antibody [EPR12539] (ab175924)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CDC40 antibody [EPR12539]
See all CDC40 primary antibodies -
Description
Rabbit monoclonal [EPR12539] to CDC40 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human CDC40 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: O60508 -
Positive control
- HeLa, K562, 293T, HUVEC cell lysates, colon tissue, transitional cell carcinoma tissue, HeLa and K562 Kcells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12539 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab175924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/1000 - 1/5000. Predicted molecular weight: 66 kDa. | |
IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
ICC/IF | 1/100 - 1/250. | |
Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Associates with the spliceosome late in the splicing pathway and may function in the second step of pre-mRNA splicing. -
Sequence similarities
Contains 7 WD repeats. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 51362 Human
- Entrez Gene: 71713 Mouse
- Entrez Gene: 361859 Rat
- Omim: 605585 Human
- SwissProt: O60508 Human
- SwissProt: Q9DC48 Mouse
- Unigene: 428147 Human
- Unigene: 46063 Mouse
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Alternative names
- Cdc40 antibody
- Cell division cycle 40 antibody
- Cell division cycle 40 homolog antibody
see all
Images
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Immunofluorescent staining of HeLa cells labeling CDC40 with ab175924 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC40 antibody [EPR12539] (ab175924)
Immunohistochemical analysis of paraffin-embedded transitional cell carcinoma tissue labeling CDC40 with ab175924 at 1/50 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC40 antibody [EPR12539] (ab175924)
Immunohistochemical analysis of paraffin-embedded colon tissue using labeling CDC40 with ab175924 at 1/50 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow cytometric analysis of permeabilized K562 cells labeling CDC40 with ab175924 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
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All lanes : Anti-CDC40 antibody [EPR12539] (ab175924) at 1/1000 dilution
Lane 1 : Hela cell lysate
Lane 2 : K562 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 66 kDa
Protocols
Datasheets and documents
References
ab175924 has not yet been referenced specifically in any publications.