Overview

  • Product name

    Cdc42 Activation Assay Kit
    See all CDC42 kits
  • Sample type

    Tissue, Adherent cells, Suspension cells
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Product overview

    Cdc42 Activation Assay Kit (ab211163) uses PAK1 PBD Agarose beads to selectively isolate and pull-down the active form of Cdc42 from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Cdc42 is detected by western blot analysis using an anti-Cdc42 specific monoclonal antibody, which reacts with the human, mouse and rat protein.


    Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.

  • Notes

    Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.  Cdc42, a 21 kDa protein, belongs to the family of Rho GTPases and regulates a variety of biological response pathways that include cell motility, cell division, gene transcription, and cell transformation.  Like other small GTPases, Cdc42 regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form.  In its active (GTP-bound) state, Cdc42 binds specifically to the p21-binding domain (PBD) of p21-activated protein kinase (PAK) to control downstream signaling cascades.

    Cdc42 is overexpressed in NSCLC (non-small cell lung cancer), colorectal adenocarcinoma, melanoma, breast cancer and testicular cancer.

Properties

Images

  • Cdc42 activation assay. Lane 1: MW Standard. Lane 2: 293 cell lysate loaded with GDP and incubated with PAK1 PBD Agarose beads. Lane 3: 293 cell lysate loaded with GTPγS and incubated with PAK1 PBD Agarose beads.

  • Detection of Cdc42 positive control (recombinant human Cdc42 from E. coli, partially purified) by immunoblot.

  • Dot blot that demonstrates specificity of anti-Cdc42 mouse antibody.

Protocols

References

ab211163 has not yet been referenced specifically in any publications.

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