Product nameAnti-CDC42 antibody
See all CDC42 primary antibodies
DescriptionRabbit polyclonal to CDC42
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human, Non human primates
Truncated Human recombinant protein, corresponding to C-terminal amino acid residues in Human CDC42 (P60953).
- A431, C6, HepG2 and HeLa cells; Human pancreas tissue; HeLa, HepG2, 293T, A431, Jurkat, MCF7, U2-OS, K652, COS7, MEF and 3T3L1 whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 30% Glycerol, 69% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab155940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 21 kDa.|
|IHC-P||1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPlasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
Sequence similaritiesBelongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
modificationsAMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
Cellular localizationCell membrane.
- Information by UniProt
FormThere are 2 isoforms produced by alternative splicing. Isoform 1 also known as: Brain; Isoform 2 also known as: Placental.
- CDC42 antibody
- CDC42_HUMAN antibody
- CDC42Hs antibody
All lanes : Anti-CDC42 antibody (ab155940) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : 293T whole cell lysate
Lane 4 : A431 whole cell lysate
Lane 5 : Jurkat whole cell lysate
Lane 6 : MCF7 whole cell lysate
Lane 7 : U2-OS whole cell lysate
Lane 8 : K652 whole cell lysate
Lane 9 : COS7 whole cell lysate
Lane 10 : MEF whole cell lysate
Lane 11 : 3T3L1 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 21 kDa
4-20% Tris HCl
Immunofluorescent analysis of A431 cells, labeling CDC42 with ab155940 at 1/20 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is also shown.
Immunofluorescent analysis of C6 cells, labeling CDC42 with ab155940 at 1/20 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is also shown.
Immunofluorescent analysis of HepG2 cells, labeling CDC42 with ab155940 at 1/200 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is also shown.
Immunofluorescent analysis of HeLa cells, labeling CDC42 with ab155940 at 1/100 dilution. Blue: Nuclei stained with Hoechst 33342.
Immunohistochemical analysis of deparaffinised Human pancreas tissue, labeling CDC42 with ab155940 at 1/20 dilution. Detection was performed using a biotin conjugated secondary antibody and SA-HRP. Right image is immunohistochemistry performed without primary antibody (negative control).
This product has been referenced in:
- Yu S et al. Mediating the invasion of smooth muscle cells into a cell-responsive hydrogel under the existence of immune cells. Biomaterials 180:193-205 (2018). Read more (PubMed: 30048909) »
- Zhao W et al. Lentivirus-mediated overexpression of CD97/ADGRE5 reverses dysregulated high glucose-induced endothelial cell migration. Mol Med Rep 15:3048-3054 (2017). WB ; Human . Read more (PubMed: 28358422) »