Key features and details
- Rabbit polyclonal to CDC42
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human, Non human primates
- Isotype: IgG
Product nameAnti-CDC42 antibody
See all CDC42 primary antibodies
DescriptionRabbit polyclonal to CDC42
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human, Non human primates
Truncated Human recombinant protein, corresponding to C-terminal amino acid residues in Human CDC42 (P60953).
- A431, C6, HepG2 and HeLa cells; Human pancreas tissue; HeLa, HepG2, 293T, A431, Jurkat, MCF7, U2-OS, K652, COS7, MEF and 3T3L1 whole cell lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 30% Glycerol (glycerin, glycerine), 69% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab155940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 21 kDa.|
|IHC-P||1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPlasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
Sequence similaritiesBelongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
modificationsAMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
Cellular localizationCell membrane.
- Information by UniProt
FormThere are 2 isoforms produced by alternative splicing. Isoform 1 also known as: Brain; Isoform 2 also known as: Placental.
- CDC42 antibody
- CDC42_HUMAN antibody
- CDC42Hs antibody
All lanes : Anti-CDC42 antibody (ab155940) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : 293T whole cell lysate
Lane 4 : A431 whole cell lysate
Lane 5 : Jurkat whole cell lysate
Lane 6 : MCF7 whole cell lysate
Lane 7 : U2-OS whole cell lysate
Lane 8 : K526 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 9 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 10 : MEF whole cell lysate
Lane 11 : 3T3L1 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 21 kDa
4-20% Tris HCl
Immunofluorescent analysis of A431 cells, labeling CDC42 with ab155940 at 1/20 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is also shown.
Immunofluorescent analysis of C6 cells, labeling CDC42 with ab155940 at 1/20 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is also shown.
Immunofluorescent analysis of HepG2 cells, labeling CDC42 with ab155940 at 1/200 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is also shown.
Immunofluorescent analysis of HeLa cells, labeling CDC42 with ab155940 at 1/100 dilution. Blue: Nuclei stained with Hoechst 33342.
Immunohistochemical analysis of deparaffinised Human pancreas tissue, labeling CDC42 with ab155940 at 1/20 dilution. Detection was performed using a biotin conjugated secondary antibody and SA-HRP. Right image is immunohistochemistry performed without primary antibody (negative control).
ab155940 has been referenced in 6 publications.
- Yu S et al. Mediating the invasion of smooth muscle cells into a cell-responsive hydrogel under the existence of immune cells. Biomaterials 180:193-205 (2018). PubMed: 30048909
- Khalkar P et al. Novel Methylselenoesters Induce Programed Cell Death via Entosis in Pancreatic Cancer Cells. Int J Mol Sci 19:N/A (2018). PubMed: 30241340
- Ransom RC et al. Mechanoresponsive stem cells acquire neural crest fate in jaw regeneration. Nature 563:514-521 (2018). PubMed: 30356216
- Zhao W et al. Lentivirus-mediated overexpression of CD97/ADGRE5 reverses dysregulated high glucose-induced endothelial cell migration. Mol Med Rep 15:3048-3054 (2017). WB ; Human . PubMed: 28358422
- Dubrac A et al. Targeting NCK-Mediated Endothelial Cell Front-Rear Polarity Inhibits Neovascularization. Circulation 133:409-21 (2016). PubMed: 26659946
- Freeman MC et al. Coronaviruses induce entry-independent, continuous macropinocytosis. MBio 5:e01340-14 (2014). Mouse . PubMed: 25096879