Overview

  • Product name
  • Description
    Rabbit polyclonal to CDC42
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Non human primates
  • Immunogen

    Truncated Human recombinant protein, corresponding to C-terminal amino acid residues in Human CDC42 (P60953).

  • Positive control
    • A431, C6, HepG2 and HeLa cells; Human pancreas tissue; HeLa, HepG2, 293T, A431, Jurkat, MCF7, U2-OS, K652, COS7, MEF and 3T3L1 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab155940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 21 kDa.
IHC-P 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

  • Function
    Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
  • Post-translational
    modifications
    AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Form
    There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: Brain; Isoform 2 also known as: Placental.
  • Alternative names
    • CDC42 antibody
    • CDC42_HUMAN antibody
    • CDC42Hs antibody
    • Cell division control protein 42 homolog antibody
    • Cell division cycle 42 (GTP binding protein 25kDa) antibody
    • Cell division cycle 42 antibody
    • dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody
    • dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody
    • G25K antibody
    • G25K GTP-binding protein antibody
    • Growth regulating protein antibody
    • GTP binding protein 25kDa antibody
    • Small GTP binding protein CDC42 antibody
    • TKS antibody
    see all

Images

  • All lanes : Anti-CDC42 antibody (ab155940) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate
    Lane 3 : 293T whole cell lysate
    Lane 4 : A431 whole cell lysate
    Lane 5 : Jurkat whole cell lysate
    Lane 6 : MCF7 whole cell lysate
    Lane 7 : U2-OS whole cell lysate
    Lane 8 : K652 whole cell lysate
    Lane 9 : COS7 whole cell lysate
    Lane 10 : MEF whole cell lysate
    Lane 11 : 3T3L1 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 21 kDa



    4-20% Tris HCl
  • Immunofluorescent analysis of A431 cells, labeling CDC42 with ab155940 at 1/20 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is also shown.
  • Immunofluorescent analysis of C6 cells, labeling CDC42 with ab155940 at 1/20 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is also shown.
  • Immunofluorescent analysis of HepG2 cells, labeling CDC42 with ab155940 at 1/200 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is also shown.
  • Immunofluorescent analysis of HeLa cells, labeling CDC42 with ab155940 at 1/100 dilution. Blue: Nuclei stained with Hoechst 33342.
  • Immunohistochemical analysis of deparaffinised Human pancreas tissue, labeling CDC42 with ab155940 at 1/20 dilution. Detection was performed using a biotin conjugated secondary antibody and SA-HRP. Right image is immunohistochemistry performed without primary antibody (negative control).

References

This product has been referenced in:
  • Yu S  et al. Mediating the invasion of smooth muscle cells into a cell-responsive hydrogel under the existence of immune cells. Biomaterials 180:193-205 (2018). Read more (PubMed: 30048909) »
  • Zhao W  et al. Lentivirus-mediated overexpression of CD97/ADGRE5 reverses dysregulated high glucose-induced endothelial cell migration. Mol Med Rep 15:3048-3054 (2017). WB ; Human . Read more (PubMed: 28358422) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Breast cancer cell, MCF-7 and MDA-MB-231)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Treatment
5nM miRNA mimics, 5nM miRNA NC, 5nM miRNA inhibitor
Specification
Breast cancer cell, MCF-7 and MDA-MB-231
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Dec 30 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Breast cancer cell: MDA-MB-231)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Breast cancer cell: MDA-MB-231
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Mr. Jinjiang Chou

Verified customer

Submitted Dec 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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