The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 21 kDa.
1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
Immunohistochemical analysis of deparaffinised Human pancreas tissue, labeling CDC42 with ab155940 at 1/20 dilution. Detection was performed using a biotin conjugated secondary antibody and SA-HRP. Right image is immunohistochemistry performed without primary antibody (negative control).
Western blot - Anti-CDC42 antibody (ab155940)
All lanes : Anti-CDC42 antibody (ab155940) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate Lane 2 : HepG2 whole cell lysate Lane 3 : 293T whole cell lysate Lane 4 : A431 whole cell lysate Lane 5 : Jurkat whole cell lysate Lane 6 : MCF7 whole cell lysate Lane 7 : U2-OS whole cell lysate Lane 8 : K652 whole cell lysate Lane 9 : COS7 whole cell lysate Lane 10 : MEF whole cell lysate Lane 11 : 3T3L1 whole cell lysate
Zhao W et al. Lentivirus-mediated overexpression of CD97/ADGRE5 reverses dysregulated high glucose-induced endothelial cell migration. Mol Med Rep15:3048-3054 (2017).
Read more (PubMed: 28358422) »
Dubrac A et al. Targeting NCK-Mediated Endothelial Cell Front-Rear Polarity Inhibits Neovascularization. Circulation133:409-21 (2016).
Read more (PubMed: 26659946) »