Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-CDC42 antibody [EPR15620] (HRP) (ab215810)

Overview

  • Product name

    Anti-CDC42 antibody [EPR15620] (HRP)
    See all CDC42 primary antibodies
  • Description

    Rabbit monoclonal [EPR15620] to CDC42 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human CDC42 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: P60953

  • Positive control

    • HeLa, HepG2, MCF7, Mouse spleen, Rat spleen, HAP1 WT (shows pos.), CDC42 HAP1 KO (shows neg.)
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab215810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.

Target

  • Function

    Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
  • Post-translational
    modifications

    AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Form

    There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: Brain; Isoform 2 also known as: Placental.
  • Alternative names

    • CDC42 antibody
    • CDC42_HUMAN antibody
    • CDC42Hs antibody
    • Cell division control protein 42 homolog antibody
    • Cell division cycle 42 (GTP binding protein 25kDa) antibody
    • Cell division cycle 42 antibody
    • dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody
    • dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody
    • G25K antibody
    • G25K GTP-binding protein antibody
    • Growth regulating protein antibody
    • GTP binding protein 25kDa antibody
    • Small GTP binding protein CDC42 antibody
    • TKS antibody
    see all

Images

  • All lanes : Anti-CDC42 antibody [EPR15620] (HRP) (ab215810) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : CDC42 knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 21 kDa


    Exposure time: 20 minutes


    ab215810 was shown to recognize CDC42 in wild-type HAP1 cells as signal was lost at the expected MW in CDC42 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CDC42 knockout samples were subjected to SDS-PAGE. ab215810 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

  • All lanes : Anti-CDC42 antibody [EPR15620] (HRP) (ab215810) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 3 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 4 : Spleen (Mouse) Tissue Lysate at 10 µg
    Lane 5 : Spleen (Rat) Tissue Lysate at 10 µg
    Lane 6 : HAP1 WT at 20 µg
    Lane 7 : CDC42 knockout HAP1 at 20 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 21 kDa
    Observed band size: 21 kDa


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab215810 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab215810 has not yet been referenced specifically in any publications.

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