Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15620] to CDC42 (HRP)
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Product nameAnti-CDC42 antibody [EPR15620] (HRP)
See all CDC42 primary antibodies
DescriptionRabbit monoclonal [EPR15620] to CDC42 (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human CDC42 aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: P60953
- HeLa, HepG2, MCF7, Mouse spleen, Rat spleen, HAP1 WT (shows pos.), CDC42 HAP1 KO (shows neg.)
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
- Anti-CDC42 antibody [EPR15620] (ab187643)
- Anti-CDC42 antibody [EPR15620] (Alexa Fluor® 488) (ab214952)
- Anti-CDC42 antibody [EPR15620] (Alexa Fluor® 647) (ab215234)
- Anti-CDC42 antibody [EPR15620] - Low endotoxin, Azide free (ab221781)
- Anti-CDC42 antibody [EPR15620] (APC) (ab225345)
- Anti-CDC42 antibody [EPR15620] (PE) (ab225346)
- HeLa whole cell lysate (ab150035)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- Mouse brain tissue lysate - total protein (ab30151)
- HT29 whole cell lysate (ab3952)
- Jurkat whole cell lysate (ab7899)
- Mouse spleen tissue lysate - total protein (ab7937)
Our Abpromise guarantee covers the use of ab215810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.|
FunctionPlasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
Sequence similaritiesBelongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
modificationsAMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
Cellular localizationCell membrane.
- Information by UniProt
FormThere are 2 isoforms produced by alternative splicing. Isoform 1 also known as: Brain; Isoform 2 also known as: Placental.
- CDC42 antibody
- CDC42_HUMAN antibody
- CDC42Hs antibody
All lanes : Anti-CDC42 antibody [EPR15620] (HRP) (ab215810) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CDC42 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 21 kDa
Exposure time: 20 minutes
ab215810 was shown to recognize CDC42 in wild-type HAP1 cells as signal was lost at the expected MW in CDC42 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CDC42 knockout samples were subjected to SDS-PAGE. ab215810 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
All lanes : Anti-CDC42 antibody [EPR15620] (HRP) (ab215810) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 4 : Spleen (Mouse) Tissue Lysate at 10 µg
Lane 5 : Spleen (Rat) Tissue Lysate at 10 µg
Lane 6 : HAP1 WT at 20 µg
Lane 7 : CDC42 knockout HAP1 at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 30 seconds
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab215810 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab215810 has not yet been referenced specifically in any publications.