Recombinant HRP Anti-CDC42 antibody [EPR15620] (ab215810)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR15620] to CDC42
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-CDC42 antibody [EPR15620]
See all CDC42 primary antibodies -
Description
HRP Rabbit monoclonal [EPR15620] to CDC42 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human CDC42 aa 50 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P60953 -
Positive control
- HeLa, HepG2, MCF7, Mouse spleen, Rat spleen, HAP1 WT (shows pos.), CDC42 HAP1 KO (shows neg.)
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15620 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-CDC42 antibody [EPR15620] (ab187643)
- Alexa Fluor® 488 Anti-CDC42 antibody [EPR15620] (ab214952)
- Alexa Fluor® 647 Anti-CDC42 antibody [EPR15620] (ab215234)
- Anti-CDC42 antibody [EPR15620] - Low endotoxin, Azide free (ab221781)
- APC Anti-CDC42 antibody [EPR15620] (ab225345)
- PE Anti-CDC42 antibody [EPR15620] (ab225346)
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- Mouse brain tissue lysate - total protein (ab30151)
- HT-29 whole cell lysate (ab3952)
- Jurkat whole cell lysate (ab7899)
- Mouse spleen tissue lysate - total protein (ab7937)
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa. |
Target
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Function
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia. -
Sequence similarities
Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily. -
Post-translational
modificationsAMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 998 Human
- Entrez Gene: 12540 Mouse
- Entrez Gene: 64465 Rat
- Omim: 116952 Human
- SwissProt: P60953 Human
- SwissProt: P60766 Mouse
- SwissProt: Q8CFN2 Rat
- Unigene: 467637 Human
see all -
Form
There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: Brain; Isoform 2 also known as: Placental. -
Alternative names
- CDC42 antibody
- CDC42_HUMAN antibody
- CDC42Hs antibody
see all
Images
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All lanes : HRP Anti-CDC42 antibody [EPR15620] (ab215810) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CDC42 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 21 kDa
Exposure time: 20 minutesab215810 was shown to recognize CDC42 in wild-type HAP1 cells as signal was lost at the expected MW in CDC42 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CDC42 knockout samples were subjected to SDS-PAGE. ab215810 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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All lanes : HRP Anti-CDC42 antibody [EPR15620] (ab215810) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 4 : Spleen (Mouse) Tissue Lysate at 10 µg
Lane 5 : Spleen (Rat) Tissue Lysate at 10 µg
Lane 6 : HAP1 WT at 20 µg
Lane 7 : CDC42 knockout HAP1 at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab215810 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab215810 has not yet been referenced specifically in any publications.