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Recombinant CDC42 (Rat)
Our Abpromise guarantee covers the use of ab41429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/125 - 1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CDC42 knockout HAP1 cell lysate (20 µg)
Lane 3: MCF7 cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab41429 observed at 20 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab41429 was shown to specifically react with CDC42 in wild-type HAP1 cells. No band was observed when CDC42 knockout samples were examined. Wild-type and CDC42 knockout samples were subjected to SDS-PAGE. ab41429 and ab181602 (loading control to GAPDH) were diluted at 1/125 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
PC-12 (Rat adrenal gland pheochromocytoma cell line) cells grown for 4 days on poly-D-lysine-coated plates in the rpesence (200 ng/ml) or absence (control) of Nerve Growth Factor (NGF) labeling CDC42 with ab41429 in ICC/IF. Primary antibody was used at 1/50 dilution followed by a donkey anti-mouse secondaty antibody (Cy2).
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