Key features and details
- Mouse monoclonal [M152] to CDC42
- Suitable for: ICC/IF, IHC-Fr, WB, ELISA
- Knockout validated
- Reacts with: Mouse, Rat, Rabbit, Human
- Isotype: IgG1
Product nameAnti-CDC42 antibody [M152]
See all CDC42 primary antibodies
DescriptionMouse monoclonal [M152] to CDC42
SpecificityThe antibody detects a 21 kDa protein in human Jurkat cells and mouse brain. It does not recognize a human RhoA GST fusion protein.
Tested applicationsSuitable for: ICC/IF, IHC-Fr, WB, ELISAmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Human
Recombinant CDC42 (Rat)
- WB: MCF7, HepG2, HAP1 and Jurkat cell lysate; Mouse brain lysate. ICC/IF: SH-SY5Y cells.
General notesDo not aliquot.
Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab41429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/125 - 1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).|
FunctionPlasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
Sequence similaritiesBelongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
modificationsAMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
Cellular localizationCell membrane.
- Information by UniProt
FormThere are 2 isoforms produced by alternative splicing. Isoform 1 also known as: Brain; Isoform 2 also known as: Placental.
- CDC42 antibody
- CDC42_HUMAN antibody
- CDC42Hs antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CDC42 knockout HAP1 cell lysate (20 µg)
Lane 3: MCF7 cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab41429 observed at 20 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab41429 was shown to specifically react with CDC42 in wild-type HAP1 cells. No band was observed when CDC42 knockout samples were examined. Wild-type and CDC42 knockout samples were subjected to SDS-PAGE. ab41429 and ab181602 (loading control to GAPDH) were diluted at 1/125 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Lanes 1 & 3 : Anti-CDC42 antibody [M152] (ab41429) at 1/125 dilution
Lanes 2 & 4 : Anti-CDC42 antibody [M152] (ab41429) at 1/500 dilution
Lanes 1-2 : human jurkat cells
Lanes 3-4 : mouse brain
Lysates/proteins at 20 µg per lane.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature.
ab41429 staining CDC42 in SH-SY5Y cells by Immunocytochemistry/ Immunofluorescence.
SH-SY5Y cells were differentiated 6 days with 10 µM retinoic acid and fixed with 4% paraformaldehyde in PBS. After 5 minutes incubation with ice-cold methanol the cells were washed twice with PBS and blocked with 10% normal donkey serum for 30 minutes. Incubation with primary antibody was performed over night at 4°C. After three-times washing with PBS, 1 hour incubation with the labeled Cy3 secondary antibody at 37°C the cells were mounted with DABCO/Mowiol.
All lanes : Anti-CDC42 antibody [M152] (ab41429) at 1/500 dilution
Lane 1 : Rabbit heart tissue lysate at 10 µg
Lane 2 : Rabbit heart tissue lysate at 20 µg
Lane 3 : Rabbit heart tissue lysate at 40 µg
Lane 4 : Rabbit heart tissue lysate at 60 µg
Lane 5 : Rabbit heart tissue lysate at 80 µg
Lane 6 : Rabbit heart tissue lysate at 100 µg
All lanes : Donkey anti-mouse IgG (HRP) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 21 kDa
PC-12 (Rat adrenal gland pheochromocytoma cell line) cells grown for 4 days on poly-D-lysine-coated plates in the rpesence (200 ng/ml) or absence (control) of Nerve Growth Factor (NGF) labeling CDC42 with ab41429 in ICC/IF. Primary antibody was used at 1/50 dilution followed by a donkey anti-mouse secondaty antibody (Cy2).
ab41429 has been referenced in 13 publications.
- López-Ureña D et al. Toxin B Variants from Clostridium difficile Strains VPI 10463 and NAP1/027 Share Similar Substrate Profile and Cellular Intoxication Kinetics but Use Different Host Cell Entry Factors. Toxins (Basel) 11:N/A (2019). PubMed: 31212980
- Zhang Q et al. miR-185 inhibits cell migration and invasion of hepatocellular carcinoma through CDC42. Oncol Lett 16:3101-3107 (2018). PubMed: 30127901
- Javadi A et al. PTEN controls glandular morphogenesis through a juxtamembrane ß-Arrestin1/ARHGAP21 scaffolding complex. Elife 6:N/A (2017). PubMed: 28749339
- Quesada-Gómez C et al. Analysis of TcdB Proteins within the Hypervirulent Clade 2 Reveals an Impact of RhoA Glucosylation on Clostridium difficile Proinflammatory Activities. Infect Immun 84:856-65 (2016). PubMed: 26755157
- Lin R et al. Electroacupuncture improves cognitive function through Rho GTPases and enhances dendritic spine plasticity in rats with cerebral ischemia-reperfusion. Mol Med Rep 13:2655-60 (2016). PubMed: 26846874