Key features and details
- Mouse monoclonal [M430] to CDC42
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgM
Product nameAnti-CDC42 antibody [M430]
See all CDC42 primary antibodies
DescriptionMouse monoclonal [M430] to CDC42
Specificityab202280 does not recognize a full length human RhoA GST fusion protein.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Dog, Pig, Drosophila melanogaster, Cynomolgus monkey
Recombinant full length protein corresponding to Rat CDC42 aa 1-191.
MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEP YTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPE ITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLK AVKYVECSALTQKGLKNVFDEAILAALEPPEPKKSRRCVLL
Database link: Q8CFN2
- Jurkat cells and mouse brain lysates; rat A7r5 cells; recombinant full length rat Cdc42 fusion protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab202280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Predicted molecular weight: 21 kDa.|
FunctionPlasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
Sequence similaritiesBelongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
modificationsAMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
Cellular localizationCell membrane.
- Information by UniProt
FormThere are 2 isoforms produced by alternative splicing. Isoform 1 also known as: Brain; Isoform 2 also known as: Placental.
- CDC42 antibody
- CDC42_HUMAN antibody
- CDC42Hs antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CDC42 knockout HAP1 cell lysate (20 µg)
Lane 3: MCF7 cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab202280 observed at 20 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab202280 was shown to recognize CDC42 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when CDC42 knockout samples were examined. Wild-type and CDC42 knockout samples were subjected to SDS-PAGE. ab202280 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-CDC42 antibody [M430] (ab202280) at 1/500 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : mouse brain lysate
Lane 3 : Recombiant full length rat Cdc42 protein
Predicted band size: 21 kDa
Immunofluorescence analysis of paraformaldehyde-fixed rat A7r5 cells, labeling CDC42 using ab202280 at a 1/200 dilution, in the absence (left, black protein block) or presence (right, red image) of full length Cdc42 recombinant protein. Goat anti-mouse conjugated to DyLight® 594 was used as secondary antibody.
ab202280 has not yet been referenced specifically in any publications.