The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Is unsuitable for WB.
May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.
Abundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 CNH domain. Contains 1 CRIB domain. Contains 1 PH domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain.
Cytoplasm. Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent.
Immunoprecipitation - Anti-CDC42 binding protein kinase alpha antibody (ab95880)
Detection of Human CDC42 binding protein kinase alpha by Immunoprecipitation, using ab95880 at 3µg/mg lysate. Image shows immunoprecipitated CDC42 binding protein kinase alpha detected with post IP WB, loading 20% of IP and using HeLa whole cell lysate at 1mg, with a95880 at 1 µg/ml in lane 1, an antibody recognising a downstream epitope in lane 2 and control IgG in lane 3. Detection: Chemiluminescence with an exposure time of 10 seconds.