Key features and details
- Rabbit polyclonal to CDC42 binding protein kinase alpha
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CDC42 binding protein kinase alpha antibody
See all CDC42 binding protein kinase alpha primary antibodies
DescriptionRabbit polyclonal to CDC42 binding protein kinase alpha
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide, corresponding to a region between residues 1610 and 1660 of Human CDC42 binding protein kinase alpha (CAH71185.1).
- HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab95886 was affinity purified using an epitope specific to CDC42 binding protein kinase alpha immobilized on solid support.
Our Abpromise guarantee covers the use of ab95886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
FunctionMay act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.
Tissue specificityAbundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 CNH domain.
Contains 1 CRIB domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm. Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent.
- Information by UniProt
- A930014J19Rik antibody
- CDC42 binding protein kinase alpha (DMPK like) antibody
- CDC42 binding protein kinase alpha antibody
Detection of CDC42 binding protein kinase alpha by Western Blot of Immunprecipitate.
An other antibody at 1µg/ml staining CDC42 binding protein kinase alpha in HeLa whole cell lysate immunoprecipitated using ab95886 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab95886 has not yet been referenced specifically in any publications.