Overview

  • Product name

    Anti-CDC42 binding protein kinase alpha antibody
    See all CDC42 binding protein kinase alpha primary antibodies
  • Description

    Rabbit polyclonal to CDC42 binding protein kinase alpha
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 373-574 of Human CDC42 binding protein kinase alpha isoform 3 (NP_055641).

  • Positive control

    • H1299, A549 and HeLa whole cell lysate (ab150035)

Properties

Applications

Our Abpromise guarantee covers the use of ab96659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 186 kDa.
IHC-P 1/100 - 1/500. Perform heat-mediated antigen retrieval with 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0)

Target

  • Function

    May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.
  • Tissue specificity

    Abundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 CNH domain.
    Contains 1 CRIB domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • Cellular localization

    Cytoplasm. Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent.
  • Information by UniProt
  • Database links

  • Alternative names

    • A930014J19Rik antibody
    • CDC42 binding protein kinase alpha (DMPK like) antibody
    • CDC42 binding protein kinase alpha antibody
    • CDC42 binding protein kinase alpha dmpk like antibody
    • CDC42 binding protein kinase beta antibody
    • CDC42-binding protein kinase alpha antibody
    • CDC42BPA antibody
    • DKFZp686L1738 antibody
    • DKFZp686P1738 antibody
    • DMPK like antibody
    • DMPK-like alpha antibody
    • FLJ23347 antibody
    • KIAA0451 antibody
    • MGC189823 antibody
    • MRCK alpha antibody
    • mrck antibody
    • mrcka antibody
    • MRCKA_HUMAN antibody
    • Myotonic dystrophy kinase related CDC42 binding protein kinase alpha antibody
    • Myotonic dystrophy kinase-related CDC42-binding kinase alpha antibody
    • Myotonic dystrophy protein kinase-like alpha antibody
    • OTTHUMP00000035727 antibody
    • OTTHUMP00000035728 antibody
    • OTTHUMP00000035729 antibody
    • OTTHUMP00000035730 antibody
    • pk428 antibody
    • RP5-1087E8.4 antibody
    • Ser thr protein kinase PK428 antibody
    • Ser thr protein kinase related to the myotonic dystrophy protein kinase antibody
    • Serine/threonine protein kinase MRCK alpha antibody
    • Serine/threonine-protein kinase MRCK alpha antibody
    see all

Images

  • Anti-CDC42 binding protein kinase alpha antibody (ab96659) at 1/1500 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 186 kDa



    7.5% SDS PAGE
  • ab96659 staining CDC42 binding protein kinase alpha in SW480 xenograft at 1/100 dilution by Immunohistochemistry (Formalin/PFA-Fixed Paraffin-embedded sections)

References

This product has been referenced in:

  • Konopaske GT  et al. Dysbindin-1 contributes to prefrontal cortical dendritic arbor pathology in schizophrenia. Schizophr Res N/A:N/A (2018). Read more (PubMed: 29759351) »
  • Unbekandt M  et al. Discovery of Potent and Selective MRCK Inhibitors with Therapeutic Effect on Skin Cancer. Cancer Res 78:2096-2114 (2018). Read more (PubMed: 29382705) »
See all 3 Publications for this product

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