Key features and details
- Rabbit polyclonal to CDC42 Binding Protein Kinase Beta
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CDC42 Binding Protein Kinase Beta antibody
DescriptionRabbit polyclonal to CDC42 Binding Protein Kinase Beta
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
Synthetic peptide within Human CDC42 Binding Protein Kinase Beta aa 750-800. The exact sequence is proprietary.
Database link: Q9Y5S2
- WB: HeLa and HEK-293T whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226345 was affinity purified using an epitope specific to CDC42 Binding Protein Kinase Beta immobilized on solid support.
Our Abpromise guarantee covers the use of ab226345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 194 kDa.|
FunctionMay act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.
Tissue specificityExpressed in all tissues examined, with high levels in heart, brain, placenta and lung.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 CNH domain.
Contains 1 CRIB domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm. Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent.
- Information by UniProt
- CDC42 binding protein kinase beta antibody
- CDC42-binding protein kinase beta antibody
- CDC42BPB antibody
All lanes : Anti-CDC42 Binding Protein Kinase Beta antibody (ab226345) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 194 kDa
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226345 has not yet been referenced specifically in any publications.