Overview

  • Product name

    Anti-CDC42 Binding Protein Kinase Beta antibody
  • Description

    Rabbit polyclonal to CDC42 Binding Protein Kinase Beta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide within Human CDC42 Binding Protein Kinase Beta aa 750-800. The exact sequence is proprietary.
    Database link: Q9Y5S2

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab226345 was affinity purified using an epitope specific to CDC42 Binding Protein Kinase Beta immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 194 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.
    • Tissue specificity

      Expressed in all tissues examined, with high levels in heart, brain, placenta and lung.
    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 CNH domain.
      Contains 1 CRIB domain.
      Contains 1 PH domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 protein kinase domain.
    • Cellular localization

      Cytoplasm. Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent.
    • Information by UniProt
    • Database links

    • Alternative names

      • CDC42 binding protein kinase beta antibody
      • CDC42-binding protein kinase beta antibody
      • CDC42BPB antibody
      • DMPK like beta antibody
      • DMPK-like beta antibody
      • KIAA1124 antibody
      • MRCK beta antibody
      • MRCKB_HUMAN antibody
      • Myotonic dystrophy kinase related CDC42 binding kinase beta antibody
      • Myotonic dystrophy kinase-related CDC42-binding kinase beta antibody
      • Myotonic dystrophy protein kinase like beta antibody
      • Myotonic dystrophy protein kinase-like beta antibody
      • Serine/threonine protein kinase MRCK beta antibody
      • Serine/threonine-protein kinase MRCK beta antibody
      see all

    Images

    • All lanes : Anti-CDC42 Binding Protein Kinase Beta antibody (ab226345) at 0.1 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 194 kDa


      Exposure time: 30 seconds


      Lysates prepared using NETN lysis buffer.

    References

    ab226345 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab226345.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up