Product nameAnti-CDC42EP5 antibody
DescriptionRabbit polyclonal to CDC42EP5
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide (Human) from the N-terminus of CDC42EP5.
- Jurkat cell extract
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab75004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 15 kDa).|
RelevanceCDC42EP5 belongs to a family of CDC42 binding proteins that induce actin filament assembly, leading to changes in cell shape.
Cellular localizationCytoplasm. Cytoskeleton. Membrane; extrinsic to membrane.
- Binder of Rho GTPase 3 like antibody
- Binder of Rho GTPases 3 antibody
- BORG3 antibody
All lanes : Anti-CDC42EP5 antibody (ab75004) at 1/500 dilution
Lane 1 : Jurkat cell extract
Lane 2 : Jurkat cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 15 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Additional bands at: 26 kDa. We are unsure as to the identity of these extra bands.
ab75004 has not yet been referenced specifically in any publications.