Anti-CDC42EP5 antibody (ab75004)
Key features and details
- Rabbit polyclonal to CDC42EP5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-CDC42EP5 antibody -
Description
Rabbit polyclonal to CDC42EP5 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide (Human) from the N-terminus of CDC42EP5.
-
Positive control
- Jurkat cell extract
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 15 kDa).
|
Notes |
---|
WB
1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 15 kDa). |
Target
-
Relevance
CDC42EP5 belongs to a family of CDC42 binding proteins that induce actin filament assembly, leading to changes in cell shape. -
Cellular localization
Cytoplasm. Cytoskeleton. Membrane; extrinsic to membrane. -
Database links
- Entrez Gene: 148170 Human
- Entrez Gene: 58804 Mouse
- Omim: 609171 Human
- SwissProt: Q6NZY7 Human
- SwissProt: Q9Z0X0 Mouse
-
Alternative names
- Binder of Rho GTPase 3 like antibody
- Binder of Rho GTPases 3 antibody
- BORG3 antibody
see all
Images
-
All lanes : Anti-CDC42EP5 antibody (ab75004) at 1/500 dilution
Lane 1 : Jurkat cell extract
Lane 2 : Jurkat cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 15 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Additional bands at: 26 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab75004 has not yet been referenced specifically in any publications.