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    cdc42ep5-antibody-ab75004.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Assembly
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Anti-CDC42EP5 antibody (ab75004)

  • Datasheet
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Western blot - Anti-CDC42EP5 antibody (ab75004)

    Key features and details

    • Rabbit polyclonal to CDC42EP5
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-CDC42EP5 antibody
    • Description

      Rabbit polyclonal to CDC42EP5
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide (Human) from the N-terminus of CDC42EP5.

    • Positive control

      • Jurkat cell extract

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS

      Without Mg2+ and Ca2+
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microfilaments
      • Actin etc
      • Actin Assembly
      • Signal Transduction
      • Signaling Pathway
      • G Protein Signaling
      • Small G Proteins
      • Regulators

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab75004 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Mouse
    Application Abreviews Notes
    WB
    1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 15 kDa).
    Notes
    WB
    1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 15 kDa).

    Target

    • Relevance

      CDC42EP5 belongs to a family of CDC42 binding proteins that induce actin filament assembly, leading to changes in cell shape.
    • Cellular localization

      Cytoplasm. Cytoskeleton. Membrane; extrinsic to membrane.
    • Database links

      • Entrez Gene: 148170 Human
      • Entrez Gene: 58804 Mouse
      • Omim: 609171 Human
      • SwissProt: Q6NZY7 Human
      • SwissProt: Q9Z0X0 Mouse
      • Alternative names

        • Binder of Rho GTPase 3 like antibody
        • Binder of Rho GTPases 3 antibody
        • BORG3 antibody
        • CDC42 effector protein (Rho GTPase binding) 5 antibody
        • CDC42 effector protein 5 antibody
        • CEP5 antibody
        see all

      Images

      • Western blot - Anti-CDC42EP5 antibody (ab75004)
        Western blot - Anti-CDC42EP5 antibody (ab75004)
        All lanes : Anti-CDC42EP5 antibody (ab75004) at 1/500 dilution

        Lane 1 : Jurkat cell extract
        Lane 2 : Jurkat cell extract with immunising peptide at 10 µg

        Lysates/proteins at 30 µg per lane.

        Predicted band size: 15 kDa
        Observed band size: 22 kDa
        why is the actual band size different from the predicted?
        Additional bands at: 26 kDa. We are unsure as to the identity of these extra bands.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab75004? Please let us know so that we can cite the reference in this datasheet.

      ab75004 has not yet been referenced specifically in any publications.

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