Overview

  • Product name
    Anti-CDC42EP5 antibody
  • Description
    Rabbit polyclonal to CDC42EP5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide (Human) from the N-terminus of CDC42EP5.

  • Positive control
    • Jurkat cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab75004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 15 kDa).
ELISA 1/40000.

Target

  • Relevance
    CDC42EP5 belongs to a family of CDC42 binding proteins that induce actin filament assembly, leading to changes in cell shape.
  • Cellular localization
    Cytoplasm. Cytoskeleton. Membrane; extrinsic to membrane.
  • Database links
    • Alternative names
      • Binder of Rho GTPase 3 like antibody
      • Binder of Rho GTPases 3 antibody
      • BORG3 antibody
      • CDC42 effector protein (Rho GTPase binding) 5 antibody
      • CDC42 effector protein 5 antibody
      • CEP5 antibody
      see all

    Images

    • Western blot - Anti-CDC42EP5 antibody (ab75004)
      Western blot - Anti-CDC42EP5 antibody (ab75004)
      All lanes : Anti-CDC42EP5 antibody (ab75004) at 1/500 dilution

      Lane 1 : Jurkat cell extract
      Lane 2 : Jurkat cell extract with immunising peptide at 10 µg

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 15 kDa
      Observed band size: 22 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 26 kDa. We are unsure as to the identity of these extra bands.

    References

    ab75004 has not yet been referenced specifically in any publications.

    Publishing research using ab75004? Please let us know so that we can cite the reference in this datasheet.

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