Key features and details
- Rabbit polyclonal to CDC45L
- Suitable for: WB, ICC/IF, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CDC45L antibody
See all CDC45L primary antibodies
DescriptionRabbit polyclonal to CDC45L
Tested applicationsSuitable for: WB, ICC/IF, IP, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human CDC45L aa 1-270.
Database link: O75419
- WB: K562, HEK-293T, HeLa and HepG2 whole cell lysate. IHC-P: Human kidney tissue. IP: K562 whole cell lysate. ICC/IF: HeLa cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 66, 58 kDa (predicted molecular weight: 66, 60, 69 kDa).|
|ICC/IF||1/50 - 1/200.|
|IP||1/20 - 1/2000.|
|IHC-P||1/20 - 1/200.|
FunctionRequired for initiation of chromosomal DNA replication.
Tissue specificityWidely expressed, highest levels are found in adult testis and thymus and in fetal liver.
Sequence similaritiesBelongs to the CDC45 family.
Developmental stageTranscript peaks at G1-S transition, but total protein remains constant throughout the cell cycle. Expressed in multiple tissues during embryogenesis, including neural crest-derived structures.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- CDC 45 antibody
- CDC 45L antibody
- CDC45 (cell division cycle 45, S.cerevisiae homolog) like antibody
All lanes : Anti-CDC45L antibody (ab230178) at 1/1000 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 66, 60, 69 kDa
Observed band size: 58,66 kDa why is the actual band size different from the predicted?
Paraffin-embedded human kidney tissue stained for CDC45L with ab230178 at 1/100 dilution in immunohistochemical analysis.
CDC45L was immunoprecipitated from K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate using ab230178.
Lane 1: Control IgG IP in K562 whole cell lysate.
Lane 2: ab230178 in K562 whole cell lysate.
Lane 3: CDC45L in K562 whole cell lysate (Input).
For western blotting, an HRP-conjugated light chain specific antibody was used as the secondary antibody (1/50,000).
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CDC45L using ab230178 at a dilution of 1/100 in ICC/IF.
Secondary is an Alexa Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230178 has not yet been referenced specifically in any publications.