For the best experience on the Abcam website please upgrade to a modern browser such as
Rabbit polyclonal to CDC45L
Recombinant fragment corresponding to Human CDC45L aa 1-270.
Database link: O75419
WB: K562, HEK-293T, HeLa and HepG2 whole cell lysate.
IHC-P: Human kidney tissue.
IP: K562 whole cell lysate.
ICC/IF: HeLa cells.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Detects a band of approximately 66, 58 kDa (predicted molecular weight: 66, 60, 69 kDa).
1/50 - 1/200.
1/20 - 1/2000.
1/20 - 1/200.
Required for initiation of chromosomal DNA replication.
Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.
Belongs to the CDC45 family.
Transcript peaks at G1-S transition, but total protein remains constant throughout the cell cycle. Expressed in multiple tissues during embryogenesis, including neural crest-derived structures.
Information by UniProt
CDC 45 antibody
CDC 45L antibody
CDC45 (cell division cycle 45, S.cerevisiae homolog) like antibody
Western blot - Anti-CDC45L antibody (ab230178)
All lanes :
Anti-CDC45L antibody (ab230178) at 1/1000 dilution
Lane 1 :
K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 :
HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 :
HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 :
HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Secondary All lanes :
Goat polyclonal to rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size:
66, 60, 69 kDa
Observed band size:
58,66 kDa (
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC45L antibody (ab230178)
Paraffin-embedded human kidney tissue stained for CDC45L with ab230178 at 1/100 dilution in immunohistochemical analysis.
Immunoprecipitation - Anti-CDC45L antibody (ab230178)
CDC45L was immunoprecipitated from K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate using ab230178.
Lane 1: Control IgG IP in K562 whole cell lysate.
Lane 2: ab230178 in K562 whole cell lysate.
Lane 3: CDC45L in K562 whole cell lysate (Input).
For western blotting, an HRP-conjugated light chain specific antibody was used as the secondary antibody (1/50,000).
Immunocytochemistry/ Immunofluorescence - Anti-CDC45L antibody (ab230178)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CDC45L using ab230178 at a dilution of 1/100 in ICC/IF.
Secondary is an Alexa Fluor
®488-conjugated Goat Anti-Rabbit IgG (H+L).
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"