Overview

  • Product name
  • Description
    Rabbit polyclonal to CDC45L
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CDC45L aa 1-270.
    Database link: O75419

  • Positive control
    • WB: K562, HEK-293T, HeLa and HepG2 whole cell lysate. IHC-P: Human kidney tissue. IP: K562 whole cell lysate. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab230178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 66, 58 kDa (predicted molecular weight: 66, 60, 69 kDa).
ICC/IF 1/50 - 1/200.
IP 1/20 - 1/2000.
IHC-P 1/20 - 1/200.

Target

  • Function
    Required for initiation of chromosomal DNA replication.
  • Tissue specificity
    Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.
  • Sequence similarities
    Belongs to the CDC45 family.
  • Developmental stage
    Transcript peaks at G1-S transition, but total protein remains constant throughout the cell cycle. Expressed in multiple tissues during embryogenesis, including neural crest-derived structures.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC 45 antibody
    • CDC 45L antibody
    • CDC45 (cell division cycle 45, S.cerevisiae homolog) like antibody
    • CDC45 antibody
    • CDC45 cell division cycle 45 like antibody
    • CDC45 like antibody
    • CDC45 related protein antibody
    • CDC45_HUMAN antibody
    • CDC45L2 antibody
    • Cell division control protein 45 homolog antibody
    • Cell division cycle 45 like 2 antibody
    • Cell division cycle 45 like antibody
    • PORC PI 1 antibody
    • PORC-PI-1 antibody
    see all

Images

  • All lanes : Anti-CDC45L antibody (ab230178) at 1/1000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 66, 60, 69 kDa
    Observed band size: 58,66 kDa (why is the actual band size different from the predicted?)

  • Paraffin-embedded human kidney tissue stained for CDC45L with ab230178 at 1/100 dilution in immunohistochemical analysis.

  • CDC45L was immunoprecipitated from K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate using ab230178.

    Lane 1: Control IgG IP in K562 whole cell lysate.

    Lane 2: ab230178 in K562 whole cell lysate.

    Lane 3: CDC45L in K562 whole cell lysate (Input).

    For western blotting, an HRP-conjugated light chain specific antibody was used as the secondary antibody (1/50,000).

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CDC45L using ab230178 at a dilution of 1/100 in ICC/IF.

    Secondary is an Alexa Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).

References

ab230178 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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