Overview

  • Product name

  • Description

    Rabbit polyclonal to CDC45L
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CDC45L aa 1-270.
    Database link: O75419

  • Positive control

    • WB: K562 and HEK-293T whole cell lysate. IHC-P: Human testis and kidney tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab230241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
WB 1/1000 - 1/5000. Detects a band of approximately 66, 58 kDa (predicted molecular weight: 66, 61, 69 kDa).

Target

  • Function

    Required for initiation of chromosomal DNA replication.
  • Tissue specificity

    Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.
  • Sequence similarities

    Belongs to the CDC45 family.
  • Developmental stage

    Transcript peaks at G1-S transition, but total protein remains constant throughout the cell cycle. Expressed in multiple tissues during embryogenesis, including neural crest-derived structures.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CDC 45 antibody
    • CDC 45L antibody
    • CDC45 (cell division cycle 45, S.cerevisiae homolog) like antibody
    • CDC45 antibody
    • CDC45 cell division cycle 45 like antibody
    • CDC45 like antibody
    • CDC45 related protein antibody
    • CDC45_HUMAN antibody
    • CDC45L2 antibody
    • Cell division control protein 45 homolog antibody
    • Cell division cycle 45 like 2 antibody
    • Cell division cycle 45 like antibody
    • PORC PI 1 antibody
    • PORC-PI-1 antibody
    see all

Images

  • All lanes : Anti-CDC45L antibody (ab230241) at 1/1000 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution

    Predicted band size: 66, 61, 69 kDa
    Observed band size: 66 kDa
    why is the actual band size different from the predicted?

  • Paraffin-embedded human testis tissue stained for CDC45L using ab230241 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human kidney tissue stained for CDC45L using ab230241 at 1/100 dilution in immunohistochemical analysis.

References

ab230241 has not yet been referenced specifically in any publications.

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